領域名 生命機能科学
教員氏名 加 藤 志 郎
研究分野 希少糖酵素工学
研究キーワード： 希少糖、微生物酵素、細菌、異性化酵素
最近の研究課題
1. 微生物酵素を用いた希少糖生産
希少糖とは「自然界に存在量の少ない単糖およびその誘導体」である。本研究では、希少糖代謝能を有する微生物を自然界から探索し、同微生物が有する酵素を用いた各種希少糖の生産を行う。
2. 異性化酵素の機能解析
糖やアミノ酸を含む構造中にキラル中心を有する化合物には互いに鏡像関係にある鏡像異性体を含む様々な異性体が存在する。このような異性体は、生体内に置いて異なる様々な生理機能を示す場合がある。本研究では、様々な異性体間の変換を触媒する酵素の探索および機能解析を実施する。
代表的な研究業績
- Kato S, Oikawa T (2017) Genome Sequence of Lactobacillus sakei LK-145 Isolated from a Japanese Sake Cellar as a High Producer of D-Amino Acids,Genome Announcements, accepted for publication.
- Kato S, Oikawa T (2017) Genome Sequence of Leuconostocmesenteroides LK-151 Isolated from a Japanese Sake Cellar as a High Producer of D-Amino Acids,Genome Announcements, accepted for publication.
- Washio T, et al. (2016) Molecular cloning and enzymological characterization of pyridoxal 5′-phosphate independent aspartate racemase from hyperthermophilic archaeon Thermococcus litoralis DSM 5473, Extremophiles, 20, 711-721.
- Kato S, et al. (2015) Enantioselective analysis of D- and L-amino acids from mouse macrophages using high performance liquid chromatography, Journal of Pharmaceutical and Biomedical Analysis, 116, 101-104.
- Kato S, et al. (2012) Lysine racemase from a lactic acid bacterium, Oenococcus oeni: Structural basis of substrate specificity, The Journal of Biochemistry, 152(6), 505-508.
- Kato S, et al. (2011) Simultaneous determination of D-amino acids by the coupling method of D-amino acid oxidase with high-performance liquid chromatography, Journal of Chromatography B, 879, 3190-3195.
Research Area: Life Science & Biotechnology
Research Specialization: Rare Sugar Enzymology
Name: KATO, Shiro
Keywords：Rare sugar, Microbial enzyme, Bacteria, Isomerase
Recent Research
1. Rare sugar production using microbial enzyme
Rare sugar is defined as monosaccharides and its derivatives that exist in the natural world only in a minute amount. Ongoing studies in our laboratory aim to develop production system of rare sugars using microbial enzymes.
2. Biochemical analysis of isomerases
Chiral compounds such as sugars and amino acids exert various physiological effects. However, their origins and functions have not been completely explained yet. Our researches focus on isomerization reactions catalyzed by various isomerases.
Publications
- Kato S, Oikawa T (2017) Genome Sequence of Lactobacillus sakei LK-145 Isolated from a Japanese Sake Cellar as a High Producer of D-Amino Acids,Genome Announcements, accepted for publication.
- Kato S, Oikawa T (2017) Genome Sequence of Leuconostocmesenteroides LK-151 Isolated from a Japanese Sake Cellar as a High Producer of D-Amino Acids,Genome Announcements, accepted for publication.
- Washio T, et al. (2016) Molecular cloning and enzymological characterization of pyridoxal 5′-phosphate independent aspartate racemase from hyperthermophilic archaeon Thermococcus litoralis DSM 5473, Extremophiles, 20, 711-721.
- Kato S, et al. (2015) Enantioselective analysis of D- and L-amino acids from mouse macrophages using high performance liquid chromatography, Journal of Pharmaceutical and Biomedical Analysis, 116, 101-104.
- Kato S, et al. (2012) Lysine racemase from a lactic acid bacterium, Oenococcus oeni: Structural basis of substrate specificity, The Journal of Biochemistry, 152(6), 505-508.
- Kato S, et al. (2011) Simultaneous determination of D-amino acids by the coupling method of D-amino acid oxidase with high-performance liquid chromatography, Journal of Chromatography B, 879, 3190-3195.
このページの管理者：管理者
