{"id":230,"date":"2017-05-25T18:14:27","date_gmt":"2017-05-25T09:14:27","guid":{"rendered":"?p=230"},"modified":"2019-08-01T11:04:54","modified_gmt":"2019-08-01T02:04:54","slug":"%e7%ac%ac11%e5%9b%9e%e5%85%ac%e9%96%8b%e3%82%bb%e3%83%9f%e3%83%8a%e3%83%bc-may-25-2017","status":"publish","type":"post","link":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/?p=230","title":{"rendered":"\u7b2c11\u56de\u516c\u958b\u30bb\u30df\u30ca\u30fc, May 25, 2017,"},"content":{"rendered":"\n

\u6f14\u984c<\/strong> \u300cMultidisciplinary interrogation of glycobiological processes for novel therapeutics\u300d<\/p>\n

\u8b1b\u5e2b\u3000<\/strong>Dr. Lingbing Kong\uff0c\u9999\u5ddd\u5927\u5b66\u56fd\u969b\u5e0c\u5c11\u7cd6\u7814\u7a76\u6559\u80b2\u6a5f\u69cb\u30fb\u52a9\u6559<\/p>\n

\u8981\u65e8\u3000<\/strong>Bacterial drug resistance has been a global grand challenge. Although scientists have endeavored for the last three decades, only a few new antimicrobials are approved by the FDA. I have initiated and led a research program that focused on the interrogation of a transporter of capsular polysaccharides Wza that is conserved in Gram-negative bacteria. Protein engineering of Wza enabled the application of single-molecule electrical channel recording to screen for blockers and understand the bi-molecular interactions at the single-molecule level. Cyclic oligosaccharide am8<\/sub>\u03b3CD that binds to the barrel of Wza with nanomolar affinity was discovered. It is non-toxic to the bacteria but it enhances the vulnerability of bacteria to immune attacks by removing the protective capsule layer. A combination of this inhibitor and a synthetic glycoconjugate vaccine based on the lipopolysaccharide has resulted in better immune clearance of bacteria from attacking the host cells. To enable the continual discovery of next-generation of Wza inhibitors, an advanced multidisciplinary single-molecule platform to study the interaction of Wza and its natural substrate K30 polysaccharide fragments has also been established. Furthermore, a novel approach to create monodisperse transmembrane alpha-helical peptide barrels has been developed, based on the C-terminal D4 domain of Wza. Overall, a combination of chemistry, biochemistry, single-molecule biophysics, biology, computation, etc. has been proven a useful and efficient approach to discover novel therapeutics and develop novel biotechnologies.<\/p>\n

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\u6f14\u984c \u300cMultidisciplinary interrogation of g Read More …<\/a><\/p>\n","protected":false},"author":1,"featured_media":231,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"_locale":"ja","_original_post":"230","footnotes":""},"categories":[5],"tags":[],"class_list":["post-230","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-5","ja"],"_links":{"self":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=\/wp\/v2\/posts\/230","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=230"}],"version-history":[{"count":2,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=\/wp\/v2\/posts\/230\/revisions"}],"predecessor-version":[{"id":261,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=\/wp\/v2\/posts\/230\/revisions\/261"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=\/wp\/v2\/media\/231"}],"wp:attachment":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=230"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=230"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/abc\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=230"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}