{"id":12805,"date":"2014-05-01T16:05:36","date_gmt":"2014-05-01T07:05:36","guid":{"rendered":"http:\/\/www.ag.kagawa-u.ac.jp?post_type=research&#038;p=12805"},"modified":"2025-04-04T14:16:59","modified_gmt":"2025-04-04T05:16:59","slug":"%e6%9d%89%e5%b1%b1%e3%80%80%e5%ba%b7%e6%86%b2","status":"publish","type":"research","link":"https:\/\/www.ag.kagawa-u.ac.jp\/?research=%e6%9d%89%e5%b1%b1%e3%80%80%e5%ba%b7%e6%86%b2","title":{"rendered":"\u6749\u5c71\u3000\u5eb7\u61b2"},"content":{"rendered":"<map name=\"reseach_img01\">\n<area href=\"https:\/\/www.ag.kagawa-u.ac.jp\/sugiyama\/TOP.html\" shape=\"rect\" coords=\"150,690,520,720\">\n<\/map>\n<map name=\"reseach_img02\">\n<area href=\"https:\/\/www.ag.kagawa-u.ac.jp\/sugiyama\/TOP.html\" shape=\"rect\" coords=\"180,715,430,740\">\n<\/map>\n\n<div class=\"wp-block-ub-tabbed-content wp-block-ub-tabbed-content-holder wp-block-ub-tabbed-content-horizontal-holder-mobile wp-block-ub-tabbed-content-horizontal-holder-tablet\" id=\"ub-tabbed-content-2830e061-5fec-45ae-aeff-a4799ab97041\">\n                <div class=\"wp-block-ub-tabbed-content-tab-holder horizontal-tab-width-mobile horizontal-tab-width-tablet\">\n                    <div role=\"tablist\" class=\"wp-block-ub-tabbed-content-tabs-title wp-block-ub-tabbed-content-tabs-title-mobile-horizontal-tab wp-block-ub-tabbed-content-tabs-title-tablet-horizontal-tab\"><div role=\"tab\" id=\"ub-tabbed-content-2830e061-5fec-45ae-aeff-a4799ab97041-tab-0\" aria-controls=\"ub-tabbed-content-2830e061-5fec-45ae-aeff-a4799ab97041-panel-0\"\n            aria-selected=\"true\" class = \"wp-block-ub-tabbed-content-tab-title-wrap active\" tabindex=\"-1\">\n            <div class=\"wp-block-ub-tabbed-content-tab-title\">\u65e5\u672c\u8a9e<\/div><\/div><div role=\"tab\" id=\"ub-tabbed-content-2830e061-5fec-45ae-aeff-a4799ab97041-tab-1\" aria-controls=\"ub-tabbed-content-2830e061-5fec-45ae-aeff-a4799ab97041-panel-1\"\n            aria-selected=\"false\" class = \"wp-block-ub-tabbed-content-tab-title-wrap\" tabindex=\"-1\">\n            <div class=\"wp-block-ub-tabbed-content-tab-title\">English<\/div><\/div><\/div><\/div>\n                <div class=\"wp-block-ub-tabbed-content-tabs-content\"><div role=\"tabpanel\" class=\"wp-block-ub-tabbed-content-tab-content-wrap active\"\n        id=\"ub-tabbed-content-2830e061-5fec-45ae-aeff-a4799ab97041-panel-0\" aria-labelledby=\"ub-tabbed-content-2830e061-5fec-45ae-aeff-a4799ab97041-tab-0\" tabindex=\"0\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:80%\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong>\u9818\u3000\u57df\u3000\u540d\u3000\u3000<mark style=\"background-color:rgba(0, 0, 0, 0);color:#000bff\" class=\"has-inline-color\">\u751f\u547d\u6a5f\u80fd\u79d1\u5b66<\/mark><\/strong><\/h2>\n\n\n\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong>\u6559\u54e1\u6c0f\u540d&nbsp;&nbsp;&nbsp;&nbsp; <mark style=\"background-color:rgba(0, 0, 0, 0);color:#0600ff\" class=\"has-inline-color\">\u6749\u3000\u5c71\u3000\u5eb7\u3000\u61b2<\/mark><\/strong><\/h2>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong>\u7814\u7a76\u5206\u91ce\u3000<mark style=\"background-color:rgba(0, 0, 0, 0);color:#0003ff\" class=\"has-inline-color\">\u52d5\u7269\u7d30\u80de\u751f\u7269\u5b66<\/mark><\/strong><\/h2>\n<\/div>\n<\/div>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"831\" height=\"12\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4.png\" alt=\"\" class=\"wp-image-28253\" style=\"width:600px;height:8px\" srcset=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4.png 831w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4-300x4.png 300w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4-768x11.png 768w\" sizes=\"auto, (max-width: 831px) 100vw, 831px\" \/><\/figure>\n\n\n\n<p class=\"has-small-font-size\"><strong>\u7814\u7a76\u30ad\u30fc\u30ef\u30fc\u30c9\uff1a\u7d30\u80de\u5185\u60c5\u5831\u4f1d\u9054\u3001\uff12\u578b\u7cd6\u5c3f\u75c5\u3001\u65b0\u898f\u89e3\u6790\u624b\u6cd5<\/strong><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:20%\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"188\" height=\"236\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-182.png\" alt=\" \u6749\u5c71\u3000\u5eb7\u61b2\" class=\"wp-image-28620\" style=\"width:141px;height:177px\"\/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong>\u6700\u8fd1\u306e\u7814\u7a76\u8ab2\u984c<\/strong><\/h2>\n\n\n\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong>\uff11\uff0e\uff12\u578b\u7cd6\u5c3f\u75c5\u306e\u7cd6\u6bd2\u6027\u306e\u89e3\u6790<\/strong><\/h2>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"has-small-font-size\">\u3000\uff12\u578b\u7cd6\u5c3f\u75c5\u306f\u751f\u6d3b\u7fd2\u6163\u3068\u907a\u4f1d\u7684\u8981\u56e0\u3067\u767a\u75c7\u3059\u308b\u75c5\u6c17\u3067\u3042\u308a\uff0c\u6162\u6027\u7684\u306a\u9ad8\u8840\u7cd6\u306b\u3088\u308b\u7cd6\u6bd2\u6027\uff08\u30a4\u30f3\u30b9\u30ea\u30f3\u5206\u6ccc\u4f4e\u4e0b\u304a\u3088\u3073\u30a4\u30f3\u30b9\u30ea\u30f3\u62b5\u6297\u6027\uff09\u306b\u3088\u308a\u75c5\u614b\u304c\u6df1\u523b\u5316\u3057\u307e\u3059\uff0e\u672c\u7814\u7a76\u5ba4\u3067\u306f\u7cd6\u5c3f\u75c5\u30e2\u30c7\u30eb\u7d30\u80de\uff08\u30e9\u30c3\u30c8\u81b5\u81d3\u30a4\u30f3\u30b9\u30ea\u30ce\u30fc\u30de\uff09\u3068\u30de\u30a6\u30b9\u306a\u3069\u306e\u5b9f\u9a13\u52d5\u7269\u3092\u7528\u3044\u3066\uff0c\u7cd6\u6bd2\u6027\u306b\u304a\u3051\u308b\u30ea\u30f3\u9178\u5316\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u306e\u7814\u7a76\u3092\u3057\u3066\u3044\u307e\u3059\uff0e\u3053\u308c\u307e\u3067\u306b\uff0c\uff12\u578b\u7cd6\u5c3f\u75c5\u306e\u7cd6\u6bd2\u6027\u306b\u304a\u3051\u308b\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u89e3\u6790\u3092\u7db2\u7f85\u7684\u306b\u884c\u3044\uff08\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u89e3\u6790\uff09\uff0c\u7cd6\u6bd2\u6027\u72b6\u614b\u306b\u304a\u3044\u3066\u69d8\u3005\u306a\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u304c\u5909\u52d5\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\uff0e\u73fe\u5728\uff0c\u3053\u308c\u3089\u306e\u907a\u4f1d\u5b50\u304c\u3069\u306e\u3088\u3046\u306b\u7cd6\u6bd2\u6027\u3092\u5236\u5fa1\u3057\u3066\u3044\u308b\u306e\u304b\u3092\u89e3\u6790\u3057\u3066\u3044\u307e\u3059\uff0e\u307e\u305f\uff0c\u7cd6\u6bd2\u6027\u306b\u304a\u3051\u308b\u5e0c\u5c11\u7cd6\u306e\u95a2\u308f\u308a\u306b\u3064\u3044\u3066\u3082\u8abf\u3079\u3066\u3044\u307e\u3059\uff0e<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"308\" height=\"338\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-183.png\" alt=\"b\u7d30\u80de\u306b\u304a\u3051\u308b\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\" class=\"wp-image-28621\" style=\"width:231px;height:253px\" srcset=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-183.png 308w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-183-273x300.png 273w\" sizes=\"auto, (max-width: 308px) 100vw, 308px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong>\uff12\uff0e\u30ea\u30f3\u9178\u5316\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u89e3\u6790\u624b\u6cd5\u306e\u958b\u767a<\/strong><\/h2>\n\n\n\n<p class=\"has-small-font-size\"> \u7d30\u80de\u5185\u304a\u3088\u3073\u7d30\u80de\u9593\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306b\u304a\u3044\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30ea\u30f3\u9178\u5316\u306f\u4e2d\u5fc3\u7684\u306a\u5f79\u5272\u3092\u62c5\u3063\u3066\u3044\u307e\u3059\uff0e\u3053\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u30ea\u30f3\u9178\u5316\u3092\u884c\u3046\u9175\u7d20\u304c\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u3067\u3059\uff0e\u30d2\u30c8\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u306f\u7d04500\u7a2e\u985e\u5b58\u5728\u3057\uff0c\u591a\u69d8\u306a\u751f\u547d\u73fe\u8c61\u3092\u5236\u5fa1\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u306b\uff0c\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u3092\u7db2\u7f85\u7684\u306b\u89e3\u6790\u3059\u308b\u305f\u3081\uff0c\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u3092\u5e45\u5e83\u304f\u691c\u51fa\u3067\u304d\u308b\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\uff08\u30de\u30eb\u30c1PK\u6297\u4f53\uff09\u3092\u4f5c\u88fd\u3057\u307e\u3057\u305f\uff0e\u52a0\u3048\u3066\uff0c\u3053\u306e\u6297\u4f53\u3092\u5229\u7528\u3057\u305f\u30ea\u30f3\u9178\u5316\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u89e3\u6790\u624b\u6cd5\u3092\u958b\u767a\u3057\u307e\u3057\u305f\uff0e\u73fe\u5728\uff0c\u65b0\u305f\u306a\u30ea\u30f3\u9178\u5316\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u89e3\u6790\u624b\u6cd5\u306e\u78ba\u7acb\u3092\u76ee\u6307\u3057\u3066\u3044\u307e\u3059\uff0e<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"307\" height=\"283\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-184.png\" alt=\"\u7cd6\u5c3f\u75c5\u30e2\u30c7\u30eb\u7d30\u80de\" class=\"wp-image-28622\" style=\"width:224px;height:206px\" srcset=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-184.png 307w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-184-300x277.png 300w\" sizes=\"auto, (max-width: 307px) 100vw, 307px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p class=\"has-text-align-center\"><strong><a href=\"http:\/\/www.ag.kagawa-u.ac.jp\/sugiyama\/TOP.html\">\u3088\u308a\u8a73\u3057\u304f\u77e5\u308a\u305f\u3044\u65b9\u306f\u3053\u3053\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/a><\/strong><\/p>\n\n\n\n<div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile\" style=\"grid-template-columns:auto 69%\"><div class=\"wp-block-media-text__content\">\n<p class=\"has-medium-font-size\"><strong>\u4ee3\u8868\u7684\u306a\u7814\u7a76\u696d\u7e3e<\/strong><\/p>\n<\/div><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"831\" height=\"12\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4.png\" alt=\"\" class=\"wp-image-28253 size-full\" srcset=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4.png 831w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4-300x4.png 300w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4-768x11.png 768w\" sizes=\"auto, (max-width: 831px) 100vw, 831px\" \/><\/figure><\/div>\n\n\n\n<ul class=\"wp-block-list has-small-font-size\">\n<li>Sugiyama. Y., <em>et al<\/em>. (2005) Generation and application of a monoclonal antibody that detects a wide variety of protein tyrosine kinases. Analytical Biochemistry 347:112-120<\/li>\n\n\n\n<li>Sugiyama. Y., <em>et al<\/em>. (2006) Two-dimensional expression pattern analysis of protein kinases after separation by MicroRotofor\/SDS\u2013PAGE. Analytical Biochemistry 359:271-273.<\/li>\n\n\n\n<li>Sugiyama. Y., <em>et al<\/em>. (2010) The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1d\/e. Biochemical journal 427:489-497.<\/li>\n\n\n\n<li>Sugiyama. Y., <em>et al<\/em>. (2011) Calcium\/calmodulin-dependent protein kinase IV involvement in the pathophysiology of glucotoxicity in rat pancreatic b-cells. Metabolism 60:145-153.<\/li>\n\n\n\n<li>Kon. N., <em>et al<\/em>. (2014) CaMKII is essential for the cellular clock and coupling between morning and evening behavioral rhythms. Genes &amp; Development 28:1101-1110.<\/li>\n\n\n\n<li>Sugiyama. Y., <em>et al<\/em>. (2015) Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE. MethodsX 2:469-474.<\/li>\n\n\n\n<li>\u7279\u8a31(2012) \u6297\u30c1\u30ed\u30b7\u30f3\u30ad\u30ca\u30fc\u30bc\u6297\u4f53\u304a\u3088\u3073\u305d\u306e\u5229\u7528\uff0c\u7279\u8a31\u7b2c4956816<\/li>\n<\/ul>\n\n<\/div><div role=\"tabpanel\" class=\"wp-block-ub-tabbed-content-tab-content-wrap ub-hide\"\n        id=\"ub-tabbed-content-2830e061-5fec-45ae-aeff-a4799ab97041-panel-1\" aria-labelledby=\"ub-tabbed-content-2830e061-5fec-45ae-aeff-a4799ab97041-tab-1\" tabindex=\"0\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:80%\">\n<h2 class=\"wp-block-heading has-medium-font-size\">Research Area:<strong>\u3000<\/strong><mark style=\"background-color:rgba(0, 0, 0, 0);color:#0004ff\" class=\"has-inline-color\">Applied Life Science<\/mark><\/h2>\n\n\n\n<h2 class=\"wp-block-heading has-medium-font-size\">Research Specialization:<strong>\u3000<\/strong><mark style=\"background-color:rgba(0, 0, 0, 0);color:#1700ff\" class=\"has-inline-color\">Animal cell biology<\/mark><strong>\u3000<\/strong><\/h2>\n\n\n\n<h2 class=\"wp-block-heading has-medium-font-size\">Name:<strong>\u3000<\/strong><mark style=\"background-color:rgba(0, 0, 0, 0);color:#0003ff\" class=\"has-inline-color\">SUGIYAMA, Yasunori<\/mark><\/h2>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"831\" height=\"12\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4.png\" alt=\"\" class=\"wp-image-28253\" style=\"width:600px;height:10px\" srcset=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4.png 831w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4-300x4.png 300w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4-768x11.png 768w\" sizes=\"auto, (max-width: 831px) 100vw, 831px\" \/><\/figure>\n\n\n\n<p class=\"has-small-font-size\">Keywords\uff1asignal transduction, type 2 diabetes, methodology<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:20%\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"188\" height=\"236\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-182.png\" alt=\"SUGIYAMA, Yasunori\" class=\"wp-image-28620\" style=\"width:130px;height:163px\"\/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong>Recent Research<\/strong><\/h2>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h2 class=\"wp-block-heading has-medium-font-size\">1.<strong>Analysis of molecular mechanisms of<\/strong> <\/h2>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-right has-medium-font-size\"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;glucotoxicity in type 2 diabetes<\/strong><\/h2>\n\n\n\n<p class=\"has-small-font-size\">Type 2 diabetes caused by poor lifestyle and genetic factor. Glucotoxicity (impaired insulin secretion and insulin resistance) induced by chronic hyperglycemia accelerates the progression of diabetes. In this laboratory, phosphorylation signaling of glucotoxicity in type 2 diabetes is analyzed by using diabetes model cells and mouse. In our previous studies, we analyzed gene expression in glucotoxicity by microarray, and found the changes in the expression of various genes. In addition, we investigate effects of rare sugars on glucotoxicity.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"308\" height=\"338\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-185.png\" alt=\"\" class=\"wp-image-28628\" style=\"width:221px;height:243px\" srcset=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-185.png 308w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-185-273x300.png 273w\" sizes=\"auto, (max-width: 308px) 100vw, 308px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong>2.\u3000Development of new analysis methods for&nbsp;&nbsp;<\/strong><\/h2>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-right has-medium-font-size\"><strong>phosphorylation signaling<\/strong><\/h2>\n\n\n\n<p class=\"has-small-font-size\">Protein phosphorylation is crucial role in cellular signal transduction. Protein kinase catalyzes phosphorylation of proteins. In the human genome, 518 protein kinase genes were identified, and the enzymes regulate diverse biological phenomena. Previous studies, we developed monoclonal antibodies, designated Multi-PK antibodies, that can recognize multiple protein kinases. Furthermore,&nbsp; the new methods for analysis of phosphorylation signaling were developed.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"361\" height=\"282\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-186.png\" alt=\"Type 2 diabetes mode cells\" class=\"wp-image-28629\" srcset=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-186.png 361w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-186-300x234.png 300w\" sizes=\"auto, (max-width: 361px) 100vw, 361px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p class=\"has-text-align-center\"><a href=\"http:\/\/www.ag.kagawa-u.ac.jp\/sugiyama\/TOP.html\">Click here&nbsp; for\u3000more information<\/a><\/p>\n\n\n\n<div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile\" style=\"grid-template-columns:auto 74%\"><div class=\"wp-block-media-text__content\">\n<p class=\"has-medium-font-size\"><strong>Publications<\/strong><\/p>\n<\/div><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"831\" height=\"12\" src=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4.png\" alt=\"\" class=\"wp-image-28253 size-full\" srcset=\"https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4.png 831w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4-300x4.png 300w, https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2022\/04\/image-4-768x11.png 768w\" sizes=\"auto, (max-width: 831px) 100vw, 831px\" \/><\/figure><\/div>\n\n\n\n<ul class=\"wp-block-list has-small-font-size\">\n<li>Sugiyama. Y., <em>et al<\/em>. (2005) Generation and application of a monoclonal antibody that detects a wide variety of protein tyrosine kinases. Analytical Biochemistry 347:112-120.<\/li>\n\n\n\n<li>Sugiyama. Y., <em>et al<\/em>. (2006) Two-dimensional expression pattern analysis of protein kinases after separation by MicroRotofor\/SDS\u2013PAGE. Analytical Biochemistry 359:271-273.<\/li>\n\n\n\n<li>Sugiyama. Y., <em>et al<\/em>. (2010) The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1d\/e. Biochemical journal 427:489-497.<\/li>\n\n\n\n<li>Sugiyama. Y., <em>et al<\/em>. (2011) Calcium\/calmodulin-dependent protein kinase IV involvement in the pathophysiology of glucotoxicity in rat pancreatic b-cells. Metabolism 60:145-153.<\/li>\n\n\n\n<li>Kon. N., <em>et al<\/em>. (2014) CaMKII is essential for the cellular clock and coupling between morning and evening behavioral rhythms. Genes &amp; Development 28:1101-1110<\/li>\n\n\n\n<li>Sugiyama. Y., <em>et al<\/em>. (2015) Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE. MethodsX 2:469-474.<\/li>\n<\/ul>\n\n<\/div><\/div>\n            <\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":15763,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","ub_ctt_via":"","footnotes":""},"class_list":["post-12805","research","type-research","status-publish","has-post-thumbnail","hentry","research_keyword-291","research_keyword-290","research_keyword-289","research_keyword-125","research_keyword-292","research_category-184","center_category-258","center_category-260","sdgs_category-6","sdgs_category-12"],"_links":{"self":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/12805","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research"}],"about":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/research"}],"author":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12805"}],"version-history":[{"count":18,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/12805\/revisions"}],"predecessor-version":[{"id":60838,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/12805\/revisions\/60838"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/media\/15763"}],"wp:attachment":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12805"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}