{"id":2415,"date":"2012-01-19T10:36:48","date_gmt":"2012-01-19T01:36:48","guid":{"rendered":"http:\/\/www.ag.kagawa-u.ac.jp?post_type=research&p=2415"},"modified":"2024-05-09T11:47:46","modified_gmt":"2024-05-09T02:47:46","slug":"%e5%b8%82%e6%9d%91%e5%92%8c%e4%b9%9f%e3%80%80%ef%bc%88%e6%a4%8d%e7%89%a9%e3%82%b2%e3%83%8e%e3%83%a0%e6%a9%9f%e8%83%bd%e8%a7%a3%e6%9e%90%e5%ad%a6%ef%bc%89","status":"publish","type":"research","link":"https:\/\/www.ag.kagawa-u.ac.jp\/?research=%e5%b8%82%e6%9d%91%e5%92%8c%e4%b9%9f%e3%80%80%ef%bc%88%e6%a4%8d%e7%89%a9%e3%82%b2%e3%83%8e%e3%83%a0%e6%a9%9f%e8%83%bd%e8%a7%a3%e6%9e%90%e5%ad%a6%ef%bc%89","title":{"rendered":"\u5e02\u6751\u3000\u548c\u4e5f"},"content":{"rendered":"\n\n<\/map>\n\n
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\u65e5\u672c\u8a9e<\/div><\/div>
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English<\/div><\/div><\/div><\/div>\n
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\u9818\u3000\u57df\u3000\u540d\u3000\u690d\u7269\u79d1\u5b66<\/mark><\/strong><\/h2>\n\n\n\n

\u6559\u54e1\u6c0f\u540d  \u5e02\u6751\u3000\u548c\u4e5f<\/mark><\/strong><\/h2>\n<\/div>\n\n\n\n
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\u7814\u7a76\u5206\u91ce\u3000\u690d\u7269\u30b2\u30ce\u30e0\u6a5f\u80fd\u89e3\u6790\u5b66<\/mark><\/strong><\/h2>\n<\/div>\n<\/div>\n\n\n\n
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\u7814\u7a76\u30ad\u30fc\u30ef\u30fc\u30c9\uff1a\u3000\u690d\u7269\u514d\u75ab\uff0c\u74b0\u5883\u30b9\u30c8\u30ec\u30b9\uff0c\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\uff0c\u9752\u67af\u75c5\u83cc\uff0c\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\uff0c\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\uff0c\u30d9\u30f3\u30b5\u30df\u30a2\u30ca\u30bf\u30d0\u30b3<\/strong><\/p>\n<\/div>\n\n\n\n

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\uff11\uff0eMAP\u30ad\u30ca\u30fc\u30bc\u7d4c\u8def\u3092\u4ecb\u3057\u305f\u690d\u7269\u514d\u75ab\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u6a5f\u69cb<\/strong><\/h2>\n\n\n\n

1.1 MEKK1\u2192MKK1\/MKK2\u2192MPK4\u7d4c\u8def\u6b20\u640d\u306b\u3088\u308b\u9632\u5fa1\u53cd\u5fdc\u8868\u73fe\u578b\u306e\u89e3\u6790<\/strong><\/h2>\n\n\n\n
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\u672c\u7814\u7a76\u5ba4\u3067\u306f\u3001\u690d\u7269\u514d\u75ab\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306e\u5206\u5b50\u6a5f\u69cb\u3092\u89e3\u660e\u3059\u308b\u305f\u3081\u3001MAMPs (\u5fae\u751f\u7269\u5206\u5b50\u30d1\u30bf\u30fc\u30f3)\u306b\u3088\u308a\u6d3b\u6027\u5316\u3059\u308b\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30caMAP\u30ad\u30ca\u30fc\u30bc\u7d4c\u8def\u306e1\u3064\u3001MEKK1\uff08MAPKKK\uff09 \u2192 MKK1\/MKK2\u3000(MAPKKs) \u2192 MPK4(MAPK) \u7d4c\u8def\u306b\u3064\u3044\u3066\u7814\u7a76\u3092\u884c\u3063\u3066\u3044\u308b\u3002\u672c\u7d4c\u8def\u306e\u6d3b\u6027\u5316\u306f\u57fa\u790e\u514d\u75ab\u3067\u3042\u308b\u30d1\u30bf\u30fc\u8a98\u5c0e\u514d\u75ab\u306b\u304a\u3051\u308b\u9632\u5fa1\u53cd\u5fdc\u3092\u6b63\u306b\u5236\u5fa1\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u304c\u3001\u7d4c\u8def\u69cb\u6210\u56e0\u5b50\u306e\u6b20\u640d\u306f\u7d30\u80de\u6b7b\u3092\u59cb\u3081\u3068\u3059\u308b\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u8a98\u5c0e\u514d\u75ab\u69d8\u9632\u5fa1\u53cd\u5fdc\u3092\u69cb\u6210\u7684\u306b\u793a\u3059\u3002\u3053\u306e\u4e00\u898b\u77db\u76fe\u3059\u308b\u73fe\u8c61\u306e\u80cc\u5f8c\u306b\u3042\u308b\u8907\u96d1\u306a\u5236\u5fa1\u6a5f\u69cb\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u305f\u3081\u3001\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u30b2\u30ce\u30e0\u60c5\u5831\u3092\u99c6\u4f7f\u3057\u3066\u907a\u4f1d\u5b66\u7684\u3001\u5206\u5b50\u751f\u7269\u5b66\u7684\u306a\u89e3\u6790\u624b\u6cd5\u3092\u53d6\u308a\u306a\u304c\u3089\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u308b\u3002<\/p>\n\n\n\n

1.2 MEKK1\u2192MKK1\/MKK2\u2192MPK4\u7d4c\u8def\u306e\u5236\u5fa1\u306b\u95a2\u4fc2\u3059\u308b\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u306e\u6a5f\u80fd<\/strong><\/p>\n\n\n\n

\u6211\u3005\u306e\u7814\u7a76\u5ba4\u3067\u306f\u3001\u4e0a\u8a18MAP\u30ad\u30ca\u30fc\u30bc\u7d4c\u8def\u306e\u7814\u7a76\u3092\u9032\u3081\u308b\u904e\u7a0b\u3067\u3001\u6700\u4e0a\u6d41\u56e0\u5b50\u3067\u3042\u308bMEKK1\u306e\u5236\u5fa1\u56e0\u5b50\u3092\u540c\u5b9a\u3059\u308b\u305f\u3081\u3001MEKK1\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u63a2\u7d22\u3092\u884c\u3063\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u3092\u5358\u96e2\u3057\u305f\u3002\u5358\u96e2\u3055\u308c\u305f\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u304c\u3001MEKK1\u4ee5\u4e0b\u306e\u7d4c\u8def\u306b\u5bfe\u3057\u3066\u3069\u306e\u3088\u3046\u306a\u6a5f\u80fd\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u306e\u304b\u3001\u5909\u7570\u4f53\u306a\u3069\u3092\u7528\u3044\u3066\u5206\u5b50\u751f\u7269\u5b66\u7684\u306b\u7814\u7a76\u3092\u884c\u3063\u3066\u3044\u308b\u3002<\/p>\n<\/div>\n\n\n\n

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1.3 \u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u306b\u57fa\u3065\u3044\u305f\u65b0\u305f\u306a\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30caMAP\u30ad\u30ca\u30fc\u30bc\u7d4c\u8def\u306e\u540c\u5b9a<\/strong><\/h2>\n\n\n\n

\u3000\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u30b2\u30ce\u30e0\u3067\u306f\u300180\u4ee5\u4e0a\u306eMAPKKK\u907a\u4f1d\u5b50\u300110\u500b\u306eMAPKK\u907a\u4f1d\u5b50\u300120\u500b\u306eMAPK\u907a\u4f1d\u5b50\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u3066\u3044\u308b\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u3001\u69d8\u3005\u306a\u7d44\u307f\u5408\u308f\u305b\u3067MAP\u30ad\u30ca\u30fc\u30bc\u7d4c\u8def\u304c\u69cb\u6210\u3055\u308c\u3001\u7a2e\u3005\u306e\u7d30\u80de\u5916\u30b7\u30b0\u30ca\u30eb\u3092\u7d30\u80de\u5185\u306b\u4f1d\u9054\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u63a8\u6e2c\u3055\u308c\u308b\u3002\u672c\u7814\u7a76\u5ba4\u3067\u306f\u3001\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30caMAP\u30ad\u30ca\u30fc\u30bc\u7d4c\u8def\u306b\u3064\u3044\u3066\u3001\u7db2\u7f85\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u7d50\u5408\u691c\u5b9a\u3092\u884c\u3044\u3001\u65b0\u5947\u306aMAP\u30ad\u30ca\u30fc\u30bc\u7d4c\u8def\u306e\u540c\u5b9a\u3092\u9032\u3081\u3066\u3044\u308b\u3002<\/p>\n\n\n\n

\uff12\uff0e\u65b0\u305f\u306a\u690d\u7269\u514d\u75ab\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u6a5f\u69cb\u540c\u5b9a\u306b\u5411\u3051\u305f\u9752\u67af\u75c5\u83cc functional effectomics<\/strong><\/h2>\n\n\n\n

\u3000\u9752\u67af\u75c5\u83cc (Ralstonia solanacearum<\/em>)\u306f\u30ca\u30b9\u79d1\u690d\u7269\u3092\u4e2d\u5fc3\u306b200\u7a2e\u985e\u4ee5\u4e0a\u306e\u690d\u7269\u306b\u5bfe\u3057\u3066\u9752\u67af\u75c5\u3092\u5f15\u304d\u8d77\u3053\u3059\u690d\u7269\u75c5\u539f\u7d30\u83cc\u3067\u3042\u308b\u3002\u9752\u67af\u75c5\u83cc\u306e\u5e83\u3044\u5bbf\u4e3b\u7bc4\u56f2\u306f\u3001\u75c5\u539f\u6027\u7d30\u83cc\u3068\u3057\u3066\u975e\u5e38\u306b\u591a\u3044\uff08>70\uff09\u75c5\u539f\u6027\u56e0\u5b50\u3067\u3042\u308b\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u306b\u8d77\u56e0\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u3002\u672c\u7814\u7a76\u3067\u306f\u9752\u67af\u75c5\u83cc\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u3092\u30c4\u30fc\u30eb\u3068\u3057\u3066\u3001\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u304c\u6291\u5236\u3059\u308b\u690d\u7269\u306e\u6a19\u7684\u56e0\u5b50\u3092\u540c\u5b9a\u3057\u3001\u65b0\u305f\u306a\u690d\u7269\u514d\u75ab\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u6a5f\u69cb\u306e\u540c\u5b9a\u3092\u76ee\u6307\u3057\u3066\u89e3\u6790\u3092\u9032\u3081\u3066\u3044\u308b\u3002<\/p>\n\n\n\n

\u3088\u308a\u8a73\u3057\u304f\u77e5\u308a\u305f\u3044\u65b9\u306f\u3053\u3053\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/a><\/strong><\/p>\n\n\n\n

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\u4ee3\u8868\u7684\u306a\u7814\u7a76\u696d\u7e3e<\/strong><\/p>\n<\/div>

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Ichimura, K. et al<\/em>.,  SGT1 contributes to maintaining protein levels of MEK2DD<\/sup> to facilitate hypersensitive response-like cell death in Nicotiana benthamiana<\/em>, Physiological and Molecular Plant Pathology<\/em>, 94, 47-52, 2016.<\/p>\n\n\n\n

Yamada, K. et al<\/em>., <\/em>The Arabidopsis<\/em> CERK1\u2010associated kinase PBL27 connects chitin perception to MAPK activation, EMBO Journal<\/em>, 35, 2468-2483, 2016.<\/p>\n\n\n\n

Nakamura, S. et al<\/em>., Mitogen-Activated Protein Kinase <\/em>Kinase<\/em> 3<\/em> Regulates Seed Dormancy in Barley, Current Biology<\/em>, 26, 775-781, 2016.<\/p>\n\n\n\n

Stegmann, M. et al. (2012) The ubiquitin ligase PUB22 targets a subunit of the exocyst complex required for PAMP-triggered responses in Arabidopsis<\/em>. Plant Cell<\/em>, 24, 4703-4716.<\/p>\n\n\n\n

Takahashi, F. et al. (2011) Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis<\/em>. Molecular Cell<\/em>, 41, 649-660.<\/p>\n\n<\/div>

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Research Area:\u3000<\/strong>Plant Science<\/mark>\u3000
<\/strong>Research Specialization: <\/strong>Plant Functional Genomics and Plant immunity<\/mark>\u3000
<\/strong>Name:\u3000<\/strong>ICHIMURA, Kazuya<\/mark><\/h2>\n\n\n\n
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Keywords: Plant immunity, Environmental stress, signal transduction, Ralstonia<\/em> <\/em>solanacearum<\/em>, effector, Arabidopsis thaliana<\/em>, Nicotiana benthamiana<\/em><\/p>\n<\/div>\n\n\n\n

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1. Plant immunity signaling mediated by MAP kinase pathways<\/strong><\/h2>\n\n\n\n
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1.1 Autoimmunity phenotype of MEKK1 \u2192 MKK1 \/ MKK2 \u2192 MPK4 pathway mutants.<\/strong><\/h2>\n\n\n\n

Arabidopsis<\/em> MEKK1, a MAPKKK, comprises specific MAPK pathway with downstream MKK1\/MKK2 (MAPKKs) and MPK4 (MAPK). This pathway is activated in the downstream of PRRs and proposed to be involved in a part of PTI signaling. In contrast, loss-of-function of the pathway results in severe dwarfism and constitutive ETI responses such as cell death and ROS accumulation. To elucidate complex mechanism underlying the phenotype, we use genetic and molecular biological approaches using Arabidopsis thaliana<\/em> genome resources.<\/p>\n<\/div>\n\n\n\n

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1.2 Ubiquitin ligase involved in regulation of <\/strong>MEKK1 <\/strong>\u2192 <\/strong>MKK1\/MKK2 <\/strong>\u2192 <\/strong>MPK4 pathway<\/strong><\/p>\n\n\n\n

We identified a ubiquitin E3 ligase as a MEKK1-binding protein by yeast two-hybrid screening. Molecular biological analyses are in progress to clarify function of E3 ligase in immunity signaling through the MEKK1\u2192 MKK1\/MKK2 \u2192 MPK4 pathway<\/p>\n<\/div>\n\n\n\n

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1.3 Identification of novel pathway related to an innate immunity singling in <\/strong>Arabidopsis<\/em><\/strong><\/strong><\/h2>\n\n\n\n

According to the annotation, Arabidopsis<\/em> genome contains over 80 MAPKKKs, 10 MAPKKs, and 20 MAPKs. This suggests that the MAP kinase pathways have to be composed of numerous combinations of protein kinases and that transduce various extracellular stimuli to activate physiological responses. We performed binding assay for the Arabidopsis<\/em> MAP kinase pathway components towards identification of a novel signaling pathway, and we analyze possible pathway to find out its biological role.<\/p>\n\n\n\n

2. Ralstonia<\/em> functional effectomics towards identifying novel immunity signaling in plants<\/strong><\/p>\n\n\n\n

Ralstonia solanacearum <\/em>is the causative agent of bacterial wilt disease against more than 200 of plant species mainly Solanaceae<\/em>. The broad host range of bacterial wilt is thought to be conferred by relatively large effector repertoire (> 70) to other pathogenic bacteria. Using the advantage of Ralstonia<\/em> effector repertoire, we performed functional screening to find effectors suppress defense responses. With these effectors, we aim to identify a novel immunity signaling components in plants.<\/p>\n\n\n\n

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Publications<\/strong><\/p>\n<\/div>

\"\"<\/figure><\/div>\n\n\n\n

Ichimura, K. et al<\/em>.,  SGT1 contributes to maintaining protein levels of MEK2DD<\/sup> to facilitate hypersensitive response-like cell death in Nicotiana benthamiana<\/em>, Physiological and Molecular Plant Pathology<\/em>, 94, 47-52, 2016.<\/p>\n\n\n\n

Yamada, K. et al<\/em>., <\/em>The Arabidopsis<\/em> CERK1\u2010associated kinase PBL27 connects chitin perception to MAPK activation, EMBO Journal<\/em>, 35, 2468-2483, 2016.<\/p>\n\n\n\n

Nakamura, S. et al<\/em>., Mitogen-Activated Protein Kinase <\/em>Kinase<\/em> 3<\/em> Regulates Seed Dormancy in Barley, Current Biology<\/em>, 26, 775-781, 2016.<\/p>\n\n\n\n

Stegmann, M. et al. (2012) The ubiquitin ligase PUB22 targets a subunit of the exocyst complex required for PAMP-triggered responses in Arabidopsis<\/em>. Plant Cell<\/em>, 24, 4703-4716.<\/p>\n\n\n\n

Takahashi, F. et al. (2011) Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis<\/em>. Molecular Cell<\/em>, 41, 649-660.<\/p>\n\n\n\n

<\/p>\n\n<\/div><\/div>\n <\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":15790,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","ub_ctt_via":"","footnotes":""},"class_list":["post-2415","research","type-research","status-publish","has-post-thumbnail","hentry","research_keyword-map","research_keyword-167","research_keyword-170","research_keyword-168","research_keyword-172","research_keyword-173","research_keyword-166","research_keyword-171","research_keyword-81","research_category-158","center_category-257","center_category-258","sdgs_category-6","sdgs_category-348","sdgs_category-12","sdgs_category-18","sdgs_category-5"],"_links":{"self":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/2415","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research"}],"about":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/research"}],"author":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2415"}],"version-history":[{"count":62,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/2415\/revisions"}],"predecessor-version":[{"id":32749,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/2415\/revisions\/32749"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/media\/15790"}],"wp:attachment":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2415"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}