{"id":3093,"date":"2012-01-23T11:24:45","date_gmt":"2012-01-23T02:24:45","guid":{"rendered":"http:\/\/www.ag.kagawa-u.ac.jp?post_type=research&p=3093"},"modified":"2024-05-09T11:42:25","modified_gmt":"2024-05-09T02:42:25","slug":"%e6%9c%a8%e6%9d%91%e7%be%a9%e9%9b%84%e3%80%80%ef%bc%88%e5%be%ae%e7%94%9f%e7%89%a9%e7%94%9f%e7%90%86%e5%ad%a6","status":"publish","type":"research","link":"https:\/\/www.ag.kagawa-u.ac.jp\/?research=%e6%9c%a8%e6%9d%91%e7%be%a9%e9%9b%84%e3%80%80%ef%bc%88%e5%be%ae%e7%94%9f%e7%89%a9%e7%94%9f%e7%90%86%e5%ad%a6","title":{"rendered":"\u6728\u6751\u3000\u7fa9\u96c4"},"content":{"rendered":"
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\u65e5\u672c\u8a9e<\/div><\/div>
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English<\/div><\/div><\/div><\/div>\n
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\u9818\u3000\u57df\u3000\u540d\u3000\u5fdc\u7528\u751f\u547d\u79d1\u5b66<\/mark><\/strong><\/h2>\n\n\n\n

\u6559\u54e1\u6c0f\u540d\u3000 \u6728\u6751\u7fa9\u96c4<\/mark>\u3000<\/strong><\/h2>\n<\/div>\n\n\n\n
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\u7814\u7a76\u5206\u91ce\u3000\u5fae\u751f\u7269\u751f\u7406\u5b66<\/mark><\/strong><\/h2>\n<\/div>\n<\/div>\n\n\n\n
\"\"<\/figure>\n\n\n\n

\u7814\u7a76\u30ad\u30fc\u30ef\u30fc\u30c9\uff1a\u7c98\u6db2\u7d30\u83cc, \u30b7\u30b0\u30ca\u30eb\u7269\u8cea, ATP\u5408\u6210,\u65b0\u898f\u5fae\u751f\u7269\u8cc7\u6e90\u3000<\/strong><\/p>\n<\/div>\n\n\n\n

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\u6700\u8fd1\u306e\u7814\u7a76\u8ab2\u984c\u3000<\/strong><\/h2>\n\n\n\n
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\uff11\uff0e\u7c98\u6db2\u7d30\u83cc\u306e\u30b7\u30b0\u30ca\u30eb\u7269\u8cea\u306b\u3064\u3044\u3066<\/strong><\/h2>\n\n\n\n

\u751f\u7269\u306f\u74b0\u5883\u306e\u5909\u5316\u304c\u8d77\u3053\u308b\u3068\u30b7\u30b0\u30ca\u30eb\u7269\u8cea\u3092\u751f\u7523\u3057\u3066\u3001\u74b0\u5883\u306e\u5909\u5316\u306b\u9069\u5fdc\u3059\u308b\u3088\u3046\u306b\u306a\u308b\u3002\u3057\u304b\u3057\u3001\u7d30\u83cc\u306b\u304a\u3051\u308b\u666e\u904d\u7684\u306a\u30b7\u30b0\u30ca\u30eb\u7269\u8cea\u3068\u3057\u3066\u306f\u30a2\u30df\u30ce\u9178\u98e2\u9913\u6642\u306b\u4f5c\u3089\u308c\u308bppGpp\u3057\u304b\u8a8d\u77e5\u3055\u308c\u3066\u3044\u306a\u3044\u3002<\/p>\n<\/div>\n\n\n\n

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\u3000\u305d\u3053\u3067\u751f\u7269\u5168\u822c\u306b\u304a\u3044\u3066\u30a2\u30df\u30ce\u9178\u98e2\u9913\u6642\u306b\u4f5c\u3089\u308c\u308b\u30a2\u30c7\u30ce\u30b7\u30f3\u30dd\u30ea\u30ea\u30f3\u9178(ApnA\uff1an=3-6)\u3068\u6570\u5341\u304b\u3089\u6570\u5343\u306e\u30ea\u30f3\u9178\u304c\u91cd\u5408\u3057\u305f\u30dd\u30ea\u30ea\u30f3\u9178\u304c\u3001\u7c98\u6db2\u7d30\u83cc\u306e\u3069\u306e\u3088\u3046\u306a\u9175\u7d20\u306b\u3088\u3063\u3066\u5408\u6210\u53ca\u3073\u5206\u89e3\u3055\u308c\u308b\u306e\u304b\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u305d\u308c\u3089\u306e\u30b7\u30b0\u30ca\u30eb\u7269\u8cea\u3092\u5408\u6210\u30fb\u5206\u89e3\u3059\u308b\u9175\u7d20\u306e\u907a\u4f1d\u5b50\u7834\u58ca\u682a\u3092\u4f5c\u88fd\u3057\u3001\u305d\u308c\u3089\u306e\u9175\u7d20\u53ca\u3073\u30b7\u30b0\u30ca\u30eb\u7269\u8cea\u306e\u6a5f\u80fd\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u3066\u7814\u7a76\u3092\u884c\u3063\u3066\u3044\u308b\u3002<\/p>\n\n\n\n

\u3000\u4e00\u65b9\u3001\u305d\u308c\u3089\u306e\u30b7\u30b0\u30ca\u30eb\u7269\u8cea\u306fATP\u306e\u5408\u6210\u57fa\u8cea\u306b\u306a\u308b\u3068\u3044\u3046\u7d50\u679c\u304c\u5f97\u3089\u308c\u3066\u304d\u3066\u3044\u308b\u306e\u3067\u3001\u3053\u308c\u3089\u3092\u57fa\u8cea\u3068\u3057\u305f\u98e2\u9913\u6642\u306eATP\u5408\u6210\u30b7\u30b9\u30c6\u30e0\u306b\u3064\u3044\u3066\u306e\u89e3\u660e\u3092\u884c\u3063\u3066\u3044\u308b\u3002<\/p>\n\n\n\n

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\uff12\uff0eSorangium<\/em>\u5c5e\u306e\u81ea\u7136\u754c\u304b\u3089\u306e\u5206\u96e2\u3068\u6709\u7528\u7269\u8cea\u306e\u63a2\u7d22<\/strong><\/h2>\n\n\n\n

\u7d30\u83cc\u306e\u4e2d\u3067\u6700\u3082\u591a\u304f\u306e\u907a\u4f1d\u5b50\u3092\u6709\u3059\u308b\u3053\u3068\u304b\u3089\u65b0\u898f\u306a\u751f\u7406\u6d3b\u6027\u7269\u8cea\u3092\u751f\u7523\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3082\u306e\u306e\u3001\u96e3\u57f9\u990a\u6027\u7d30\u83cc\u3067\u3042\u308b\u305f\u3081\u7d14\u5316\u304c\u96e3\u3057\u304f\u3001\u65e5\u672c\u3067\u306f\u5206\u96e2\u3055\u308c\u3066\u3044\u306a\u3044\u7c98\u6db2\u7d30\u83cc\u306e\u5206\u96e2\u53ca\u3073\u7d14\u5316\u65b9\u6cd5\u3092\u78ba\u7acb\u3057\u3001\u653e\u7dda\u83cc\u306b\u66ff\u308f\u308b\u65b0\u3057\u3044\u5fae\u751f\u7269\u8cc7\u6e90\u3092\u69cb\u7bc9\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u3066\u81ea\u7136\u754c\u304b\u3089\u7c98\u6db2\u7d30\u83cc\u3092\u5206\u96e2\u3057\u3066\u3044\u308b\u3002\u7c98\u6db2\u7d30\u83cc\u306e\u83cc\u682a\u3068\u57f9\u990a\u62bd\u51fa\u7269\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u4f5c\u88fd\u3059\u308b\u3053\u3068\u3067\u3001\u4f01\u696d\u7b49\u306e\u5171\u540c\u7814\u7a76\u306b\u304a\u3044\u3066\u5275\u85ac\u306e\u305f\u3081\u306e\u65b0\u898f\u30ea\u30fc\u30c9\u5316\u5408\u7269\u306e\u63a2\u7d22\u3092\u884c\u3044\u305f\u3044\u3002<\/p>\n<\/div>\n\n\n\n

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\u4ee3\u8868\u7684\u306a\u7814\u7a76\u696d\u7e3e<\/strong><\/p>\n<\/div>

\"\"<\/figure><\/div>\n\n\n\n
    \n
  • Kimura, Y. et al. (2017). High concentrations of intracellular Ap4A and\/or Ap5A in developing Myxococcus xanthus<\/em> cells inhibit sporulation. Microbiology 163:86-93.<\/li>\n\n\n\n
  • Oka, M. et al. (2016). Lysyl-tRNA synthetase from Myxococcus xanthus<\/em> catalyzes the formation of diadenosine penta- and\u3000hexaphosphates from adenosine tetraphosphate. Archieves of Biochemistry and Biophysics. 604:152-158.<\/li>\n\n\n\n
  • Sasaki, M. et al. (2014). Enzymatic characteristics of an ApaH-like phosphatase, PrpA, and a diadenosine tetraphosphate hydrolase, ApaH, from Myxococcus xanthus<\/em> enzymes. FEBS Letters 588:3395-3402.<\/li>\n\n\n\n
  • Kimura, Y. et al. (2012). Function analysis of a bacterial tyrosine kinase, BtkB, in Myxococcus xanthus<\/em>. FEMS<\/em> Microbiological Letters<\/em> 336(1):45-51 (2012).<\/li>\n\n\n\n
  • Mori, Y. et al. (2012). PhpA, a tyrosine phosphatase of Myxococcus xanthus<\/em>, is involved in the production of exopoly-saccharide. Microbiology<\/em>158(10):2546-2555.<\/li>\n\n\n\n
  • Kimura, Y. et al. (2011). A Myxococcus xanthus<\/em> bacterial tyrosine kinase, BtkA, is required for the formation of mature spores. Journal of Bacteriology<\/em> 193(20):5853-5857.<\/li>\n<\/ul>\n\n<\/div>
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    Research Area:\u3000<\/strong>Applied Life Science<\/mark><\/h2>\n\n\n\n

    Research Specialization:\u3000<\/strong>MicrobialPhysiology<\/mark>\u3000<\/strong><\/h2>\n\n\n\n

    Name:\u3000<\/strong>KIMURA, Yoshio<\/mark><\/h2>\n\n\n\n
    \"\"<\/figure>\n\n\n\n

    Keywords: Myxobacteria, signal transduction, ATP synthesis,  Novel microbial resources<\/p>\n<\/div>\n\n\n\n

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    \"\"<\/figure>\n<\/div>\n<\/div>\n\n\n\n

    Recent Research<\/strong><\/h2>\n\n\n\n
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    1.  Signal molecules of Myxobacteria.<\/strong><\/h2>\n\n\n\n

      When environmental conditions become unfavorable,organisms produce signal molecules and can respond to changes in their environment.  Diadenosine polyphosphate (Apn<\/sub>A; n=3-6) and polyphosphateare produced during amino acid-starvation. We try to identify and characterize key enzymes involved in these signal molecules of synthesis and degradation. Further, we generate  gene-broken strains in which the enzyme genes are broken, and we will clarify the function of these enzymes and signal molecules.<\/p>\n<\/div>\n\n\n\n

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      On the other hand, we are revealing that these signal molecules are substrates for ATP synthesis enzymes. We now studying that ATP synthesis system using these signal molecules under amino acid-starvation.<\/p>\n\n\n\n

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    2. Isolation of Sorangium<\/em> from the natural world andSearch for useful substances.<\/strong><\/h2>\n\n\n\n

    \u3000Gram-negative myxobacteria are an important source of novel classes of secondary metabolites. Of these, the genus Sorangium<\/em> is particularly valuable. Therefore, we are isolating the genus Sorangium<\/em> form soil.<\/p>\n\n\n\n

       Natural products are extracted from Sorangium<\/em> cultures, and are provided for microbial growth inhibition experiments. In addition, the extracts are analyzed by LC-MS.    \u3000<\/p>\n\n\n\n

      <\/strong> we are exploring useful substances from these cultures of Sorangium<\/em>.<\/p>\n<\/div>\n\n\n\n

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    Publications<\/strong><\/p>\n<\/div>

    \"\"<\/figure><\/div>\n\n\n\n
      \n
    • Kimura, Y. et al. (2017). High concentrations of intracellular Ap4A and\/or Ap5A in developing Myxococcus xanthus<\/em> cells inhibit sporulation. Microbiology 163:86-93.<\/li>\n\n\n\n
    • Oka, M. et al. (2016). Lysyl-tRNA synthetase from Myxococcus xanthus<\/em> catalyzes the formation of diadenosine penta- and\u3000hexaphosphates from adenosine tetraphosphate. Archieves of Biochemistry and Biophysics. 604:152-158.<\/li>\n\n\n\n
    • Sasaki, M. et al. (2014). Enzymatic characteristics of an ApaH-like phosphatase, PrpA, and a diadenosine tetraphosphate hydrolase, ApaH, from Myxococcus xanthus<\/em> enzymes. FEBS Letters 588:3395-3402.<\/li>\n\n\n\n
    • Kimura, Y. et al. (2012). Function analysis of a bacterial tyrosine kinase, BtkB, in Myxococcus xanthus<\/em>. FEMS<\/em> Microbiological Letters<\/em> 336(1):45-51 (2012).<\/li>\n\n\n\n
    • Mori, Y. et al. (2012). PhpA, a tyrosine phosphatase of Myxococcus xanthus<\/em>, is involved in the production of exopoly-saccharide. Microbiology<\/em>158(10):2546-2555.<\/li>\n\n\n\n
    • Kimura, Y. et al. (2011). A Myxococcus xanthus<\/em> bacterial tyrosine kinase, BtkA, is required for the formation of mature spores. Journal of Bacteriology<\/em> 193(20):5853-5857.<\/li>\n<\/ul>\n\n<\/div><\/div>\n <\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":15782,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","ub_ctt_via":"","footnotes":""},"class_list":["post-3093","research","type-research","status-publish","has-post-thumbnail","hentry","research_category-184","center_category-260","sdgs_category-12"],"_links":{"self":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3093","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research"}],"about":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/research"}],"author":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3093"}],"version-history":[{"count":46,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3093\/revisions"}],"predecessor-version":[{"id":32744,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3093\/revisions\/32744"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/media\/15782"}],"wp:attachment":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3093"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}