{"id":3200,"date":"2012-01-23T15:15:54","date_gmt":"2012-01-23T06:15:54","guid":{"rendered":"http:\/\/www.ag.kagawa-u.ac.jp?post_type=research&p=3200"},"modified":"2024-05-08T15:24:05","modified_gmt":"2024-05-08T06:24:05","slug":"%e6%9c%ab%e5%90%89-%e7%b4%80%e8%a1%8c%e3%80%80%ef%bc%88%e5%88%86%e5%ad%90%e7%b4%b0%e8%83%9e%e7%94%9f%e7%89%a9%e5%ad%a6%ef%bc%89-2","status":"publish","type":"research","link":"https:\/\/www.ag.kagawa-u.ac.jp\/?research=%e6%9c%ab%e5%90%89-%e7%b4%80%e8%a1%8c%e3%80%80%ef%bc%88%e5%88%86%e5%ad%90%e7%b4%b0%e8%83%9e%e7%94%9f%e7%89%a9%e5%ad%a6%ef%bc%89-2","title":{"rendered":"\u672b\u5409\u3000\u7d00\u884c"},"content":{"rendered":"\n\n<\/map>\n\n
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\u9818\u3000\u57df\u3000\u540d\u3000\u3000\u751f\u547d\u6a5f\u80fd\u79d1\u5b66<\/mark><\/strong><\/h2>\n\n\n\n

\u6559\u54e1\u6c0f\u540d\u3000\u3000 \u672b \u5409  \u7d00 \u884c\u3000<\/mark><\/strong><\/h2>\n<\/div>\n\n\n\n
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\u7814\u7a76\u5206\u91ce\u3000\u5206\u5b50\u7d30\u80de\u751f\u7269\u5b66<\/mark><\/strong><\/h2>\n<\/div>\n<\/div>\n\n\n\n
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\u7814\u7a76\u30ad\u30fc\u30ef\u30fc\u30c9\uff1a\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u3001\u30d7\u30ed\u30c6\u30a4\u30f3\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u3001\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5<\/strong><\/p>\n<\/div>\n\n\n\n

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\u6700\u8fd1\u306e\u7814\u7a76\u8ab2\u984c<\/strong><\/h2>\n\n\n\n
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1. \u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u306e\u521d\u671f\u767a\u751f\u306b\u95a2\u4e0e\u3059\u308b<\/strong> \u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000 \u3000\u30bf\u30f3\u30d1\u30af\u8cea\u30ea\u30f3\u9178\u5316\u5236\u5fa1\u5206\u5b50<\/strong><\/mark>\u3000<\/strong><\/h2>\n\n\n\n

\u3000\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30ea\u30f3\u9178\u5316\u306f\u3001\u7d30\u80de\u5185\u60c5\u5831\u4f1d\u9054\u7d4c\u8def\u306b\u304a\u3044\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6a5f\u80fd\u3092\u8fc5\u901f\u306b\u8abf\u7bc0\u3059\u308b\u91cd\u8981\u306a\u6a5f\u69cb\u3067\u3042\u308b\u3002\u810a\u690e\u52d5\u7269\u306e\u521d\u671f\u767a\u751f\u904e\u7a0b\u306b\u304a\u3044\u3066\u3082\u3001\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u3068\u30d7\u30ed\u30c6\u30a4\u30f3\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u3092\u4ecb\u3057\u3066\u5de7\u5999\u306b\u5236\u5fa1\u3055\u308c\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u3001\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u3092\u30e2\u30c7\u30eb\u52d5\u7269\u3068\u3057\u3066\u7528\u3044\u3001\u521d\u671f\u306e\u5f62\u614b\u5f62\u6210\u306b\u95a2\u4e0e\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u30ea\u30f3\u9178\u5316\u5236\u5fa1\u5206\u5b50\u306b\u3064\u3044\u3066\u89e3\u6790\u3057\u3066\u304a\u308a\u3001\u8edf\u9aa8\u3068\u80f8\u9c2d\u306e\u5f62\u6210\u306b\u95a2\u4e0e\u3059\u308b\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bcCaMKId\u3084\u3001\u795e\u7d4c\u7d30\u80de\u306e\u751f\u5b58\u306b\u5fc5\u9808\u3067\u3042\u308b\u30d7\u30ed\u30c6\u30a4\u30f3\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bcCaMKP-N\u306a\u3069\u3092\u898b\u51fa\u3057\u305f\u3002<\/p>\n<\/div>\n\n\n\n

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\uff12\uff0e\u30d7\u30ed\u30c6\u30a4\u30f3\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u306e\u6d3b\u6027\u5236\u5fa1\u6a5f\u69cb<\/mark><\/strong><\/h2>\n\n\n\n

\u3000\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u3001<\/strong>\u3000\u6570\u3042\u308b\u30d7\u30ed\u30c6\u30a4\u30f3\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u306e\u4e2d\u3067\u3082\u3001\u6d3b\u6027\u306e\u767a\u73fe\u306b\uff12\u4fa1\u306e\u91d1\u5c5e\u30a4\u30aa\u30f3\u3092\u5fc5\u8981\u3068\u3059\u308bPPM\u30d5\u30a1\u30df\u30ea\u30fc\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u306b\u7740\u76ee\u3057\u3066\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u308b\u3002\u7279\u306b\u3001CaM\u30ad\u30ca\u30fc\u30bc\u306b\u7279\u7570\u7684\u306b\u4f5c\u7528\u3059\u308bCaMKP\/PPM1F\u3068\u3001\u305d\u306e\u6838\u5c40\u5728\u578bCaMKP-N\/PPM1E\u306f\u5f53\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u304c\u767a\u898b\u3057\u305f\u5206\u5b50\u3067\u3042\u308a\u3001\u3053\u308c\u3089\u306b\u7d50\u5408\u3057\u3066\u6d3b\u6027\u3092\u8abf\u7bc0\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u63a2\u7d22\u3084\u3001\u7d30\u80de\u5185\u30d7\u30ed\u30bb\u30b7\u30f3\u30b0\u3084\u30ea\u30f3\u9178\u5316\u3092\u4ecb\u3057\u305f\u6d3b\u6027\u8abf\u7bc0\u6a5f\u69cb\u306e\u89e3\u660e\u3092\u9032\u3081\u3066\u3044\u308b\u3002<\/p>\n<\/div>\n\n\n\n

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\u3088\u308a\u8a73\u3057\u304f\u77e5\u308a\u305f\u3044\u65b9\u306f\u3053\u3053\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/mark><\/a><\/strong><\/p>\n\n\n\n

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\u4ee3\u8868\u7684\u306a\u7814\u7a76\u696d\u7e3e<\/strong><\/p>\n<\/div>

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  • Senga and Akizuki et al. <\/em>High-performance CaMKI: A highly active and stable form of CaMKId produced by high-level  soluble expression in Escherichia coli.<\/em> Biochem. Biophys. Res. Commun. <\/em>475, 277-282, 2016<\/li>\n\n\n\n
  • Onouchi et al. <\/em>Regulation of Ca2+<\/sup>\/calmodulin-dependent protein kinase phosphatase (CaMKP\/PPM1F) by Protocadherin-gC5(Pcdh-gC5). Arch. Biochem. Biophys. <\/em>585, 109-120, 2015<\/li>\n\n\n\n
  • Senga et al. <\/em>Expression and gene knockdown of zebrafish Ca2+<\/sup>\/calmodulin-dependent protein kinase I\u03b4-LL. Arch. Biochem.Biophys. <\/em>540, 41-52, 2013<\/li>\n\n\n\n
  • Sueyoshi et al. <\/em>Functional processing of nuclear Ca2+<\/sup>\/calmodulin-dependent protein kinase phosphatase (CaMKP-N):  evidence for a critical role of proteolytic processing in the regulation of its catalytic activity, subcellular localization and substrate targeting in vivo<\/em>. Arch. Biochem. Biophys. <\/em>517, 43-52, 2012<\/li>\n\n\n\n
  • Senga et al. <\/em>Knockdown of two splice variants of Ca2+<\/sup>\/calmodulin-dependent protein kinase I\u03b4 causes developmental abnormalities in zebrafish, Daniorerio<\/em>. Arch. Biochem. Biophys. <\/em>517, 71-82, 2012<\/li>\n\n\n\n
  • Onouchi et al. <\/em>Phosphorylation and activation of nuclear Ca2+<\/sup>\/calmodulin-dependent protein kinase phosphatase (CaMKP-N\/PPM1E) by Ca2+<\/sup>\/calmodulin-dependent protein kinase I (CaMKI). Biochem. Biophys. Res. Commun. <\/em>422, 703-709, 2012<\/li>\n\n\n\n
  • Baba et al. <\/em>Regulation of Ca2+<\/sup>\/calmodulin-dependent protein kinase phosphatase (CaMKP) by oxidation\/reduction at Cys-359. Arch. Biochem. Biophys. <\/em>526, 9-15, 2012<\/li>\n<\/ul>\n\n<\/div>
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    Research Area:\u3000\u3000Life Science & Biotechnology<\/mark>\u3000\u3000\u3000\u3000
    Research Specialization:\u3000Molecular and Cellular Biology\u3000<\/mark>
    Name: SUEYOSHI, Noriyuki<\/mark><\/strong><\/h2>\n\n\n\n
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    KeyWords\uff1aProtein Kinase, Protein Phosphatase, Zebrafish<\/p>\n<\/div>\n\n\n\n

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    Recent Research<\/strong><\/h2>\n\n\n\n
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    1. Functions of protein kinases and phosphatases on zebrafish embryogenesis.\u3000<\/mark><\/strong><\/h2>\n\n\n\n

    Protein kinases and phosphatases are known to play pivotal roles in various signaling pathways and to participate in diverse cellular processes including proliferation, development and differentiation. To investigate protein kinases and phosphatases involved in the developmental processes in zebrafish embryogenesis, we are performing functional gene knockdown experiments in zebrafish using antisense morpholino-modified oligonucleotides that is targeted to the 5\u2019-noncoding sequences of various protein kinases and phosphatases.<\/p>\n<\/div>\n\n\n\n

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    \uff12\uff0eRegulation of PPM family protein phosphatases.<\/mark><\/strong><\/h2>\n\n\n\n

    \u3000<\/strong> Ca2<\/sup>+<\/sup>\/CaM-dependent protein kinase phosphatase (CaMKP) is a Ser\/Thr protein phosphatase that belongs to the PPM family. In our laboratory, we focus on the regulation mechanisms of PPM family phosphatases, especially of <\/strong>CaMKP<\/strong> and <\/strong>CaMKP<\/strong>-N, a nuclear isoform of <\/strong>CaMKP<\/strong>.<\/strong>\u3000\u3000<\/strong><\/p>\n<\/div>\n\n\n\n

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    Publications<\/strong><\/p>\n<\/div>

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      \n
    • Senga and Akizuki et al. <\/em>High-performance CaMKI: A highly active and stable form of CaMKId produced by high-level  soluble expression in Escherichia coli.<\/em> Biochem. Biophys. Res. Commun. <\/em>475, 277-282, 2016<\/li>\n\n\n\n
    • Onouchi et al. <\/em>Regulation of Ca2+<\/sup>\/calmodulin-dependent protein kinase phosphatase (CaMKP\/PPM1F) by Protocadherin-gC5(Pcdh-gC5). Arch. Biochem. Biophys. <\/em>585, 109-120, 2015<\/li>\n\n\n\n
    • Senga et al. <\/em>Expression and gene knockdown of zebrafish Ca2+<\/sup>\/calmodulin-dependent protein kinase I\u03b4-LL. Arch. Biochem.Biophys. <\/em>540, 41-52, 2013<\/li>\n\n\n\n
    • Sueyoshi et al. <\/em>Functional processing of nuclear Ca2+<\/sup>\/calmodulin-dependent protein kinase phosphatase (CaMKP-N):  evidence for a critical role of proteolytic processing in the regulation of its catalytic activity, subcellular localization and substrate targeting in vivo<\/em>. Arch. Biochem. Biophys. <\/em>517, 43-52, 2012<\/li>\n\n\n\n
    • Senga et al. <\/em>Knockdown of two splice variants of Ca2+<\/sup>\/calmodulin-dependent protein kinase I\u03b4 causes developmental abnormalities in zebrafish, Daniorerio<\/em>. Arch. Biochem. Biophys. <\/em>517, 71-82, 2012<\/li>\n\n\n\n
    • Onouchi et al. <\/em>Phosphorylation and activation of nuclear Ca2+<\/sup>\/calmodulin-dependent protein kinase phosphatase (CaMKP-N\/PPM1E) by Ca2+<\/sup>\/calmodulin-dependent protein kinase I (CaMKI). Biochem. Biophys. Res. Commun. <\/em>422, 703-709, 2012<\/li>\n\n\n\n
    • Baba et al. <\/em>Regulation of Ca2+<\/sup>\/calmodulin-dependent protein kinase phosphatase (CaMKP) by oxidation\/reduction at Cys-359. Arch. Biochem. Biophys. <\/em>526, 9-15, 2012<\/li>\n<\/ul>\n\n<\/div><\/div>\n <\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":15779,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","ub_ctt_via":"","footnotes":""},"class_list":["post-3200","research","type-research","status-publish","has-post-thumbnail","hentry","research_keyword-206","research_keyword-207","research_keyword-205","research_keyword-204","research_keyword-171","research_category-184","center_category-260","sdgs_category-6","sdgs_category-12"],"_links":{"self":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3200","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research"}],"about":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/research"}],"author":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3200"}],"version-history":[{"count":49,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3200\/revisions"}],"predecessor-version":[{"id":32646,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3200\/revisions\/32646"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/media\/15779"}],"wp:attachment":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}