{"id":3299,"date":"2012-01-23T17:06:23","date_gmt":"2012-01-23T08:06:23","guid":{"rendered":"http:\/\/www.ag.kagawa-u.ac.jp?post_type=research&p=3299"},"modified":"2024-05-09T11:37:35","modified_gmt":"2024-05-09T02:37:35","slug":"%e7%94%b0%e6%b7%b5%e5%85%89%e6%98%ad%ef%bc%88%e5%bf%9c%e7%94%a8%e5%88%86%e5%ad%90%e7%b4%b0%e8%83%9e%e7%94%9f%e7%89%a9%e5%ad%a6%ef%bc%89","status":"publish","type":"research","link":"https:\/\/www.ag.kagawa-u.ac.jp\/?research=%e7%94%b0%e6%b7%b5%e5%85%89%e6%98%ad%ef%bc%88%e5%bf%9c%e7%94%a8%e5%88%86%e5%ad%90%e7%b4%b0%e8%83%9e%e7%94%9f%e7%89%a9%e5%ad%a6%ef%bc%89","title":{"rendered":"\u7530\u6df5\u3000\u5149\u662d"},"content":{"rendered":"
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\u9818 \u57df \u540d \u751f\u547d\u6a5f\u80fd\u79d1\u5b66<\/mark><\/h2>\n\n\n\n

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1. \u9175\u6bcd\u907a\u4f1d\u5b66\u306b\u3088\u308b\u819c\u30b9\u30c8\u30ec\u30b9\u5fdc\u7b54\u30fb\u4fee\u5fa9\u6a5f\u69cb\u306e\u89e3\u6790<\/h2>\n\n\n\n

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\u3000\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u3001\u5358\u7d30\u80de\u771f\u6838\u751f\u7269\u3067\u3042\u308b\u9175\u6bcd\u3092\u7528\u3044\u3066\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u306e\u6a5f\u80fd\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u30b7\u30b9\u30c6\u30e0\u3092\u958b\u767a\u3057\u3001\u672c\u30b7\u30b9\u30c6\u30e0\u3092\u7528\u3044\u3066\u3053\u308c\u307e\u3067\u306b\u9752\u67af\u75c5\u83cc\u3068\u547c\u3070\u308c\u308b\u690d\u7269\u75c5\u539f\u83cc\u304c\u6709\u3059\u308b\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u306e\u5206\u5b50\u6a5f\u80fd\u306e\u89e3\u660e\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u308b\u3002
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\u4ee3\u8868\u7684\u306a\u7814\u7a76\u696d\u7e3e<\/h2>\n<\/div>
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  1. \u85e4\u539f\u7965\u5b50,\u7530\u6df5\u5149\u662d<\/span>.* \u690d\u7269\u75c5\u539f\u83cc\u306e\u201c\u72e1\u733e\u3055”\u3092\u9175\u6bcd\u3067\u89e3\u304f:\u30b0\u30eb\u30bf\u30c1\u30aa\u30f3\u3092\u5206\u89e3\u3059\u308b\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u306e\u767a\u898b\u3068\u305d\u306e\u6a5f\u80fd\u89e3\u6790,\u5316\u5b66\u3068\u751f\u7269\uff0c 54<\/strong>(12)\uff0c869-870\uff0c2016.<\/li>\n\n\n\n
  2. Popa C.\uff0cTabuchi M<\/span>.and Valls M. Modification of bacterial effector proteins inside eukaryotic host cells. Front. Cell<\/em>.Infect. Microbiol., 6<\/strong>\uff0c73\uff0c2016.<\/li>\n\n\n\n
  3. Fujiwara S,Kawazoe T.\uff0cOhnishi K.\uff0cKitagawa T., Popa C,. Valls M.,Genin S.\uff0cNakamura K.\uff0cKuramitsu Y.\uff0cTanaka N. and Tabuchi M<\/span>.RipAY, a plant pathogen effector protein, exhibits robust \u03b3-glutamyl cyclotransferase activity when stimulated by eukaryotic thioredoxins. J. Biol. Chem<\/em>., 291<\/strong>(13),6813-6830,2016.<\/li>\n\n\n\n
  4. Tabuchi M<\/span>., Audhya A., Parsons A.B, Boone C., Emr S.D. The phosphatidylinositol 4,5-biphosphate and TORC2 binding proteins Slm1 and Slm2 function in sphingolipid regulation.Mol. Cell Biol<\/em>.15<\/strong>,5861-75,2006.<\/li>\n<\/ol>\n\n<\/div>
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    Research Area: Applied Life Science<\/mark>
    Research Specialization: Applied Molecular Cell Biology<\/mark>
    Name: TABUCHI , Mitsuaki<\/mark><\/h2>\n\n\n\n
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    KEYWORDs: Yeast, eisosome, Stress response, Pathogen effector<\/p>\n<\/div>\n\n\n\n

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    1.Plasma Membrane Stress Response in Yeast<\/h2>\n\n\n\n

    \u3000Plasma membrane is constitutively damaged from various physical stresses such as heat stress,osmotic stress, membrane tension, which causes plasma membrane stresses. For example, in humans,myocytes are constantly damaged by exposure to physical membrane stress by repeating expansion and contraction as they move intensely. However, cells have a mechanism to repair such damage. In animal cells, it is known that a structure called “Caveolae” plays an essential role in repairing membrane damage, and caveolae abnormality is known to be a cause of cardiomyopaty.<\/p>\n\n\n\n

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    \u3000Yeast is a unicellular eukaryote and has contributed to the A) Yeast Eisosomes are
    visualized with red elucidation fvarious intracellular mechanisms such as autophagy as fluorescent protein\u00b7tagged a mod e1 eu ka ryo1:\u00b7t c ce1 1 . M ech aru\u00b7 sms 1+’0 r sens\u00b7m g and repa1\u00b7 n\u00b7 ng Pmiilclr ousncdoepry .t he fluorescent membrane stress are also present in yeast cells, and “Eisosome” are known as organelles corresponding to caveolae of animal cells. Our laboratory focus on cellular fimction of eisosome and is interested in the downstream signaling pathway that activates the membrane repair during plasma membrane stress.<\/p>\n<\/div>\n\n\n\n

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    2.Functional Analysis of Pathogen Effector Proteins in Yeast<\/h2>\n\n\n\n
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    \u3000Numerous bacterial pathogens of plants and animals inject virulence proteins, so-called effetors,directory into the host cell cytoplasm hrough specialized secretion apparatuses, such as the type \u2162 secretion system. The translocated effector proteins manipulate diverse host cellular processes to promote the perturbationof host immune system and ultimately cause infection.
    \u3000In our laboratory, we are conducting yeast system to uncover the molecular fimction of effectors from Ralstonia solanacarum, which is the most destructive bacterial pathogen in plant. If the effector inhibits the yeast counterpart of the host effector target and then give us a phenotype, such as growth inhibition of yeast. This effector-induced growth inhibition allows us to understand the molecular function of effectors. Recently, by using the yeast system, we found one of the R. solanacearum effector, RipAY fimctions as a glutathione degradation enzyme to inhibit host immune response (1, 2).<\/p>\n<\/div>\n\n\n\n

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    Publications<\/p>\n<\/div>

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    1. Pop a C., Tabuchi M<\/span>. and Valls M. Modification of bacterial effector proteins inside eukaryotic host cells. Front. Cell.Inject. JvJi.crobioL, 6, 73, 2016.<\/li>\n\n\n\n
    2. Fujiwara S., Kawazoe T., Ohnishi K., Kitagawa T., Pop a C., Valls M., Genin S., Nakamura K., Kuramitsu Y., Tanaka N.and Tabuchi M<\/span>. Rip A Y, a p !ant pathogen effector protein, exhibits robust y-glutamy I eye lotransferase activity when stimulated by eukaryotic thioredoxins. J. BioL Chern., 291 (13), 6813-6830, 2016.<\/li>\n\n\n\n
    3. Tabuchi M<\/span>., AudhyaA., Parsons A.B., Boone C., Emr S.D. The phosphatidylinositol4,5-biphosphate and TORC2 binding proteins Slm1 and Slm2 function in sphingolipid regulation. Mol. Cell Bioi. 15, 5861-75, 2006 .<\/li>\n<\/ol>\n\n<\/div><\/div>\n <\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":15777,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_uag_custom_page_level_css":"","ub_ctt_via":"","footnotes":""},"class_list":["post-3299","research","type-research","status-publish","has-post-thumbnail","hentry","research_keyword-211","research_keyword-212","research_keyword-214","research_keyword-213","research_category-184","center_category-260","center_category-259","sdgs_category-6","sdgs_category-12","sdgs_category-5"],"uagb_featured_image_src":{"full":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi.jpg",288,200,false],"thumbnail":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi-150x150.jpg",150,150,true],"medium":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi.jpg",288,200,false],"medium_large":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi.jpg",288,200,false],"large":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi.jpg",288,200,false],"1536x1536":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi.jpg",288,200,false],"2048x2048":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi.jpg",288,200,false],"gb-block-post-grid-landscape":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi.jpg",288,200,false],"gb-block-post-grid-square":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi.jpg",288,200,false],"post-thumbnail":["https:\/\/www.ag.kagawa-u.ac.jp\/wp-content\/uploads\/2012\/01\/tabuchi-288x198.jpg",288,198,true]},"uagb_author_info":{"display_name":"\u7ba1\u7406\u8005","author_link":"https:\/\/www.ag.kagawa-u.ac.jp\/?author=1"},"uagb_comment_info":0,"uagb_excerpt":null,"_links":{"self":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3299","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research"}],"about":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/research"}],"author":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3299"}],"version-history":[{"count":31,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3299\/revisions"}],"predecessor-version":[{"id":32738,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3299\/revisions\/32738"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/media\/15777"}],"wp:attachment":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3299"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}