{"id":3435,"date":"2012-01-24T16:23:47","date_gmt":"2012-01-24T07:23:47","guid":{"rendered":"http:\/\/www.ag.kagawa-u.ac.jp?post_type=research&p=3435"},"modified":"2024-05-09T11:14:36","modified_gmt":"2024-05-09T02:14:36","slug":"%e5%b7%9d%e6%9d%91%e3%80%80%e7%90%86%e3%80%80%ef%bc%88%e9%a3%9f%e5%93%81%e8%a1%9b%e7%94%9f%e5%ad%a6%ef%bc%89","status":"publish","type":"research","link":"https:\/\/www.ag.kagawa-u.ac.jp\/?research=%e5%b7%9d%e6%9d%91%e3%80%80%e7%90%86%e3%80%80%ef%bc%88%e9%a3%9f%e5%93%81%e8%a1%9b%e7%94%9f%e5%ad%a6%ef%bc%89","title":{"rendered":"\u5ddd\u6751\u3000\u7406"},"content":{"rendered":"
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\u65e5\u672c\u8a9e<\/div><\/div>
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English<\/div><\/div><\/div><\/div>\n
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\u9818\u3000\u57df\u3000\u540d\u3000\u3000\u98df\u54c1\u79d1\u5b66<\/mark><\/strong><\/h2>\n\n\n\n

\u6559\u54e1\u6c0f\u540d\u3000\u3000 \u5ddd\u3000\u6751\u3000\u3000\u7406<\/mark><\/strong><\/h2>\n<\/div>\n\n\n\n
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\u7814\u7a76\u5206\u91ce\u3000\u98df\u54c1\u885b\u751f\u5b66<\/mark><\/strong><\/h2>\n<\/div>\n<\/div>\n\n\n\n
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\u7814\u7a76\u30ad\u30fc\u30ef\u30fc\u30c9\uff1a\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3(\u30ab\u30d3\u6bd2)\u3001\u30de\u30a4\u30af\u30ed\u30b7\u30b9\u30c1\u30f3(\u30a2\u30aa\u30b3\u6bd2\uff09\u3001\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3001\u30a4\u30e0\u30ce\u30a2\u30d5\u30a3\u30cb\u30c6\u30fc\u30ab\u30e9\u30e0\u3001\uff25\uff2c\uff29\uff33\uff21\u3001\u98df\u306e\u5b89\u5168<\/strong><\/p>\n<\/div>\n\n\n\n

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\u6700\u8fd1\u306e\u7814\u7a76\u8ab2\u984c<\/strong><\/h2>\n\n\n\n
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\"\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\uff08\u30ab\u30d3\u6bd2\uff09\u306b\u3088\u308b\u98df\u54c1\u6c5a\u67d3\u3068\u5065\u5eb7\u88ab\u5bb3\u306e\u6982\u8981\"<\/figure>\n<\/div>\n<\/div>\n\n\n\n

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\"\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u306e\u4f5c\u88fd\u306e\u6982\u8981\"<\/figure>\n<\/div>\n<\/div>\n\n\n\n

\uff12\uff0e\u30de\u30a4\u30af\u30ed\u30b7\u30b9\u30c1\u30f3(\u30a2\u30aa\u30b3\u6bd2\uff09\u306b\u5bfe\u3059\u308b\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u306e\u4f5c\u88fd\u3068\u74b0\u5883\u6c34\u6c5a\u67d3\u8abf\u67fb<\/strong><\/h2>\n\n\n\n
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\u3000\u30de\u30a4\u30af\u30ed\u30b7\u30b9\u30c1\u30f3(MC\uff09\u306f\u5bcc\u6804\u990a\u5316\u3057\u305f\u6e56\u6cbc\u3067\u767a\u751f\u3059\u308b\u30a2\u30aa\u30b3\u304c\u4f5c\u308b\u6bd2\u7d20\u306e1\u3064\u3067\u3001\u6700\u3082\u6bd2\u6027\u306e\u5f37\u3044\u306e\u304cMC-LR\u3067\u3042\u308b\u3002\u6d77\u5916\u3067\u306fMC\u6c5a\u67d3\u6c34\u306e\u6442\u53d6\u3067\u5bb6\u755c\u3084\u30d2\u30c8\u306e\u5065\u5eb7\u88ab\u5bb3\u304c\u767a\u751f\u3057\u3066\u3044\u308b\u3002\u9999\u5ddd\u770c\u306b\u306f1,400\u4ee5\u4e0a\u306e\u6e9c\u6c60\u304c\u3042\u308a\u3001\u590f\u5b63\u306b\u306f\u591a\u304f\u306e\u6e9c\u3081\u6c60\u3067\u30a2\u30aa\u30b3\u304c\u767a\u751f\u3057\u3066\u3044\u308b(\u56f3\uff13\uff09\u3002\u3057\u304b\u3057\u3001MC\u306e\u6c5a\u67d3\u8abf\u67fb\u306f\u307b\u3068\u3093\u3069\u884c\u308f\u308c\u3066\u3044\u306a\u3044\u3002\u305d\u3053\u3067\u3001\u9999\u5ddd\u306e\u6e9c\u6c60\u306eMC\u6c5a\u67d3\u30ec\u30d9\u30eb\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u306b\u3001MC-LR\u306b\u5bfe\u3059\u308b\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3092\u4f5c\u88fd\u3057\u3001WHO\u306e\u98f2\u6599\u6c34\u306e\u57fa\u6e96 1 \u00b5g\/L\u306e1\/10\u4ee5\u4e0b\u306e80 ng\/L\u307e\u3067\u306eMC-LR\u3092\u6e2c\u5b9a\u3067\u304d\u308bELISA\u6cd5\u3092\u78ba\u7acb\u3057\u305f\u3002\u73fe\u5728\u3001\u672c\u6cd5\u3092\u7528\u3044\u3066\u9999\u5ddd\u306e\u6e9c\u3081\u6c60\u306e\u6c5a\u67d3\u8abf\u67fb\u3092\u5b9f\u65bd\u3057\u3066\u304a\u308a\u3001\u4e00\u90e8\u306e\u6e9c\u6c60\u306eMC\u9ad8\u6fc3\u5ea6\u6c5a\u67d3\u3092\u660e\u3089\u304b\u306b\u3057\u305f\u3002<\/p>\n<\/div>\n\n\n\n

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\"\u9999\u5ddd\u5927\u5b66\u8fb2\u5b66\u90e8\u306e\u8fd1\u90ca\u3001\u7537\u4e95\u9593\u6c60\u3067\u767a\u751f\u3057\u305f\u30a2\u30aa\u30b3(2013\u5e748\u6708\uff09\"<\/figure>\n<\/div>\n<\/div>\n\n\n\n
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\u4ee3\u8868\u7684\u306a\u7814\u7a76\u696d\u7e3e<\/strong><\/p>\n<\/div>

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  • A. T. Ishikawa, O. Kawamura et. al.<\/em>, , Exposure Assessment of Infants to Aflatoxin M1<\/sub> through Consumption of Breast Milk and Infant Powdered Milk in Brazil. Toxins<\/em>, 8, 246-256 (2016)<\/li>\n\n\n\n
  • \u7530\u6e15\u7d50\u5b9f\u3001\u5ddd\u6751\u3000\u7406\uff1a\u9999\u5ddd\u5927\u5b66\u8fb2\u5b66\u90e8\u8fd1\u90ca\u306e\u6e9c\u6c60\uff0c\u7537\u4e95\u9593\u6c60\u3068\u5e73\u7530\u6c60\u306e\u30de\u30a4\u30af\u30ed\u30b7\u30b9\u30c1\u30f3\u6c5a\u67d3\u8abf\u67fb(2014)\u3001\u9999\u5ddd\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a\u300069\uff0c23-26 (2017).<\/li>\n\n\n\n
  • \u5149\u5143\u7d50\u82b1\u3001\u5ddd\u6751\u3000\u7406\uff1a\u8b83\u5c90\u3046\u3069\u3093\u8abf\u7406\u4e2d\u306e\u30aa\u30af\u30e9\u30c8\u30ad\u30b7\u30f3A\u306e\u6319\u52d5\u3068\u5e02\u8ca9\u8b83\u5c90\u3046\u3069\u3093\uff65\u305d\u3046\u3081\u3093\u4e2d\u306e\u30aa\u30af\u30e9\u30c8\u30ad\u30b7\u30f3A\u306e\u6c5a\u67d3\u8abf\u67fb\u3001\u9999\u5ddd\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a\u300069\uff0c27-30 (2017).<\/li>\n<\/ul>\n\n\n\n

    <\/p>\n\n<\/div>

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    Research Area:\u3000Food Science\u3000<\/mark>
    <\/strong>Research Specialization:\u3000Food Hygiene<\/mark>
    <\/strong>Name: KAWAMURA, Osamu<\/mark><\/strong><\/h2>\n\n\n\n
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    Keywords\uff1aMycotoxin, Microcystine (algae toxin), Monoclonal antibody, Immunoaffinity column, ELISA, Food safety<\/strong><\/p>\n<\/div>\n\n\n\n

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    Recent Research<\/strong><\/h2>\n\n\n\n
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       Mycotoxins, the toxic second metabolites of some fungi, give healthy damage to humans and domestic animals. These fungi are infected with the crops in farms, and the fungi grow on per-\/post-harvest crops and produce mycotoxins. Mycotoxins remain on the crops after extinction of the fungi, and are heat stable compounds, therefore after cooking, remain in foods. Furthermore, it is known that mycotoxins in animal feeds are transferred to livestock products. A variety of food is contaminated with small quantity of mycotoxins. By an intake of these food for a long term, it is thought that mycotoxins cause the chronic poisoning such as “cancers”.<\/p>\n<\/div>\n\n\n\n

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    1. Production of monoclonal antibodies against mycotoxins, Development of the immunochemical analysis for mycotoxins in foods, Food contamination investigation, and Risk evaluation<\/strong><\/h2>\n\n\n\n
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       To make clear contamination level of mycotoxins in foods, a simple and easy and high sensitive analysis methods are necessary. Now, the immunochemical methods using antibody are frequently used. We made monoclonal antibodies (mAb) against major mycotoxins, and established immunoaffinity column-HPLC methods using these mAb.<\/p>\n\n\n\n

       We analyzed mycotoxins in commercial foods, such as rice, coffee, cocoa, and ramen from Japan and Asian countries and feeds for chicken from Brazil and Indonesia We performed behavior experiments of mycotoxin during food processing. We obtained some new findings.<\/p>\n<\/div>\n\n\n\n

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    2. Occurrence of algae toxin (microcystine) in fresh water<\/strong><\/h2>\n\n\n\n

    Microcystin-Leu-Arg (MC-LR) is one of toxic cyanotoxins, which are contaminated with eutrophicated lakes and ponds. We established a sensitive ELISA method for detection of MC-LR in environmental water using our novel mAb. Our best MC.5-3 mAb was a little strongly reacted to MC-RR than MC-LR, and almost same to MC-YR. The sensitivity of our ELISA was 80 pg\/mL of MC-LR. This value was less than 1\/10 of the regulation level (1 ng\/mL) in drinking water by WHO. Using this ELISA, we perform the pollution investigation into reservoirs of Kagawa.<\/p>\n<\/div>\n\n\n\n

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    Publications<\/strong><\/p>\n<\/div>

    \"\"<\/figure><\/div>\n\n\n\n
      \n
    • A. T. Ishikawa, O. Kawamura<\/span> et.al.<\/em>;  Exposure Assessment of Infants to Aflatoxin M1<\/sub> through Consumption of Breast Milk and Infant Powdered Milk in Brazil. Toxins<\/em>, 8, 246-256 (2016)<\/li>\n\n\n\n
    • Y. Tbuchi & O. Kawamura<\/span>; Occurrence of microcystins in Oima Pond and Hirata Pond which were reservoirs in the suburbs of Faculty of Agriculture, Kagawa University. (2014)\u3001Technical Bulletin of Faculty of Agriculture, Kagawa University, 69\uff0c23-26 (2017).<\/li>\n\n\n\n
    • Y. Mistumoto & O. Kawamura<\/span>; Decrease of ochratoxin A during cooking of Sanuki udon and occurrence of ochratoxin A in commercial Sanuki udon and somen, Technical Bulletin of Faculty of Agriculture, Kagawa University, 69\uff0c27-30 (2017).<\/li>\n<\/ul>\n\n<\/div><\/div>\n <\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":15774,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","ub_ctt_via":"","footnotes":""},"class_list":["post-3435","research","type-research","status-publish","has-post-thumbnail","hentry","research_keyword-222","research_keyword-223","research_keyword-195","research_keyword-224","research_category-217","center_category-258","center_category-259","sdgs_category-6","sdgs_category-5"],"_links":{"self":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3435","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research"}],"about":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/research"}],"author":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3435"}],"version-history":[{"count":26,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3435\/revisions"}],"predecessor-version":[{"id":32734,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/research\/3435\/revisions\/32734"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=\/wp\/v2\/media\/15774"}],"wp:attachment":[{"href":"https:\/\/www.ag.kagawa-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3435"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}