Publications

Peer-Reviewed Papers

（Bold : Lab member,  * Corresponding author）

 

Synthesis of Unnatural Aplysiatoxin Analogs with High Affinity for Protein Kinase C.

Mana Morishita, Kohei Hada, Yusuke Araki, Keisuke Fukaya, Daisuke Urabe, Ryo C. Yanagita, Jumpei Maki, Yusuke Hanaki, Masaki Kita, Kazuhiro Irie, Toshio Nishikawa*.

Eur. J. Org. Chem. 2025, in press.

 

Production of rare sugars by transketolase in combination with ketose 3-epimerase.

Akihide Yoshihara*, Yusuke Hanaki, Susumu Mochizuki, Hiromi Yoshida, Ken Izumori, Shigehiro Kamitori.

Methods Enzymol. 2025, in press.

 

Synthetic Study of 8- and 9-Carbon Sugars by Transaldolase.

Akihide Yoshihara*, Erika Miyoshi, Shunsuke Tomino, Yusuke Hanaki, Susumu Mochizuki, Hiromi Yoshida, Ken Izumori, Shigehiro Kamitori*.

J. Agric. Food Chem. 2025, 73 (30), 18914–18922.

 

Efficient production of D-sedoheptulose using transketolase from Thermus thermophilus HB8 and epimerization reaction using D-tagatose 3-epimerase from Pseudomonas cichorii ST24.

Kana Watanuki, Susumu Mochizuki, Yusuke Hanaki, Hiromi Yoshida, Shigehiro Kamitori, Ken Izumori, Akihide Yoshihara*.

Biosci. Biotechnol. Biochem. 2025, 89 (9), 1279–1285.

 

Six-step synthesis and epithelial–mesenchymal transition-inhibitory activity of a tetralone-based vitetrifolin analog.

Yusuke Hanaki*, Yasunori Sugiyama, Ryo C. Yanagita.

Biosci. Biotechnol. Biochem. 2025, 89 (6), 805–810.

 

Identification of lotus cultivar-specific rhizome compounds and evaluation of thier growth inhibitory activity against Fusarium commune.

Yusuke Hanaki*, Yasumasa Hara, Miu Mori, Keita Tomiyoshi.

Fundam. Toxicol. Sci. 2025, 12 (1), 1–5.

 

Effect of phenolic-hydroxy-group incorporation on the biological activity of a simplified aplysiatoxin analog with an (R)-(–)-carvone-based core.

Ryo C. Yanagita*, Yoshiyuki Suzuki, Yasuhiro Kawanami, Yusuke Hanaki, Kazuhiro Irie.

Biosci. Biotechnol. Biochem. 2024, 88 (9), 992–998.

 

A new labdane-type diterpenoid from leaves of Vitex rotundifolia.

Yusuke Hanaki*, Rintaro Abe, Yasunori Sugiyama, Yasumasa Hara, Ryo C. Yanagita.

Results Chem. 2024, 7, 101512.

 

Biological evaluation of a phosphate ester prodrug of 10-methyl-aplog-1, a simplified analogue of aplysiatoxin, as a possible latency-reversing agent for HIV reactivation.

Jumpei Maki, Yusuke Hanaki, Ryo C. Yanagita, Masayuki Kikumori, Anastasiia Kovba, Ayaka Washizaki, Chihiro Tsukano, Hirofumi Akari, Kazuhiro Irie*.

Biosci. Biotechnol. Biochem. 2023, 87 (12), 1453–1461.

 

In vivo anti-cancer activity of 10-methyl-aplog-1, a simplified analog of aplysiatoxin, and its possible signaling pathway associated with G1 arrest.

Yusuke Hanaki, Yuki Shikata, Masayuki Kikumori, Mutsumi Okamura, Shingo Dan, Masaya Imoto, Kazuhiro Irie*.

Biochem. Biophys. Res. Commun. 2023, 675, 19–25.

 

Cell Morphology-Based Screening Identified Vitetrifolin D from Vitex Rotundifolia as an Inhibitor of Phorbol Ester-Induced Downregulation of E-Cadherin in HHUA Endometrial Cells.

Yusuke Hanaki*, Nichika Iwase, Yasunori Sugiyama, Sena Miyoshi, Ryo C. Yanagita.

BPB Reports 2023, 6 (3), 103-107.

 

A simplified analog of debromoaplysiatoxin lacking the B-ring of spiroketal moiety retains protein kinase C-binding and antiproliferative activities.

Tomoki Sekido, Kosuke Yamamoto, Ryo C. Yanagita*, Yasuhiro Kawanami, Yusuke Hanaki, Kazuhiro Irie.

Bioorg. Med. Chem. 2022, 73, 116988.

 

12-O-Tetradecanoylphorbol 13-acetate promotes proliferation and epithelial–mesenchymal transition in HHUA cells cultured on collagen type I gel: A feasible model to find new therapies for endometrial diseases.

Yusuke Hanaki*, Sena Miyoshi, Yasunori Sugiyama, Ryo C. Yanagita, Masashi Sato.

Biosci. Biotechnol. Biochem. 2022, 86 (10), 1417-1422. [Accepted Manuscript]

 

Design, synthesis, and biological activity of synthetically accessible analog of aplysiatoxin with (R)-(–)-carvone-based conformation-controlling unit.

Yoshiyuki Suzuki, Keiichi Moritoki, Mizuki Kajiwara, Ryo C. Yanagita*, Yasuhiro Kawanami, Yusuke Hanaki, Kazuhiro Irie.

Biosci. Biotechnol. Biochem. 2022, 86 (8), 1013-1023.

 

Analysis of binding mode of vibsanin A with protein kinase C C1 domeins: An experimental and molecular dynamics simulation study.

Ryo C. Yanagita*, Mao Otani, Satoshi Hatanaka, Hiroto Nishi, Shota Miyake, Yusuke Hanaki, Masashi Sato, Yasuhiro Kawanami, Kazuhiro Irie.

J. Mol. Struct. 2022, 1260, 132866.

 

Oscillatoxin E and Its C7 Epimer Show Distinct Growth Inhibition Profiles against Several Cancer Cell Lines.

Yusuke Hanaki*, Yusuke Araki, Toshio Nishikawa, Ryo C. Yanagita.

Heterocycles 2021, 102 (12), 2353-2362.

 

Evaluation of the in vitro cytotoxicity of oscillatoxins E and F under nutrient-starvation culture conditions.

Yusuke Hanaki*, Yusuke Araki, Toshio Nishikawa, Ryo C. Yanagita.

Fundam. Toxicol. Sci. 2021, 8 (3), 69-73.

 

 

Before Kagawa Univ.

 

Stereodivergent Attached-Ring Synthesis via Non-Covalent Interactions: A Short Formal Synthesis of Merrilactone A.

Benjamin J. Huffman, Tiffany Chu, Yusuke Hanaki, Jonathan Wong, Shuming Chen, Ken Houk, Ryan A. Shenvi*.

Angew. Chem. Int. Ed. 2022, 61 (3), e202114514.

 

Total synthesis and biological evaluation of oscillatoxins D, E, and F.

Yusuke Araki^†, Yusuke Hanaki^† (^†equal contribution), Masaki Kita, Koutaro Hayakawa, Kazuhiro Irie, Yoshihiko Nokura, Atsuo Nakazaki, Toshio Nishikawa*.

Biosci. Biotechnol. Biochem. 2021, 85 (6), 1371-1382.

 

Synthesis and Biological Activities of Acetal Analogs at Position 3 of 10-Methyl-Aplog-1, a Potential Anti-Cancer Lead Derived from Debromoaplysiatoxin.

Koutaro Hayakawa, Yusuke Hanaki, Harukuni Tokuda, Ryo C. Yanagita, Yu Nakagawa, Mutsumi Okamura, Shingo Dan, Kazuhiro Irie*.

Heterocycles 2018, 97 (1), 478-492.

 

Identification of protein kinase C isozymes involved in the anti-proliferative and pro-apoptotic activities of 10-Methyl-aplog-1, a simplified analog of debromoaplysiatoxin, in several cancer cell lines.

Yusuke Hanaki, Yuki Shikata, Masayuki Kikumori, Natsuki Hotta, Masaya Imoto, Kazuhiro Irie*.

Biochem. Biophys. Res. Commun. 2018, 495 (1), 438-445.

 

Loss of the Phenolic Hydroxyl Group and Aromaticity from the Side Chain of Anti-Proliferative 10-Methyl-Aplog-1, a Simplified Analog of Aplysiatoxin, Enhances Its Tumor-Promoting and Proinflammatory Activities.

Yusuke Hanaki, Masayuki Kikumori, Harukuni Tokuda, Mutsumi Okamura, Shingo Dan, Naoko Adachi, Naoaki Saito, Ryo C. Yanagita, Kazuhiro Irie*.

Molecules 2017, 22 (4), 631.

 

Synthetic Models of Quasi-Stable Amyloid β40 Oligomers with Significant Neurotoxicity.

Yumi Irie, Kazuma Murakami, Mizuho Hanaki, Yusuke Hanaki, Takashi Suzuki, Yoko Monobe, Tomoyo Takai, Ken-ichi Akagi, Kazuhiro Irie*.

ACS Chem. Neurosci. 2017, 8 (4), 807-816.

 

Synthesis and biological activities of the amide derivative of aplog-1, a simplified analog of aplysiatoxin with anti-proliferative and cytotoxic activities.

Yusuke Hanaki, Ryo C. Yanagita, Takahiro Sugahara, Misako Aida, Harukuni Tokuda, Nobutaka Suzuki, Kazuhiro Irie*.

Biosci. Biotechnol. Biochem. 2015, 79 (6), 888-895.

 

Two New Lyngbyatoxin Derivatives from the Cyanobacterium, Moorea Producens.

Weina Jiang, Satoshi Tan, Yusuke Hanaki, Kazuhiro Irie, Hajime Uchida, Ryuichi Watanabe, Toshiyuki Suzuki, Bryan Sakamoto, Michiya Kamio, Hiroshi Nagai*.

Marine Drugs 2014, 12 (12), 5788-5800.

 

Structure–activity studies at position 27 of aplog-1, a simplified analog of debromoaplysiatoxin with anti-proliferative activity.

Yusuke Hanaki, Masayuki Kikumori, Sayo Ueno, Harukuni Tokuda, Nobutaka Suzuki, Kazuhiro Irie*.

Tetrahedron 2013, 69 (36), 7636-7645.