研究業績

<学術論文>
[2020年]
Yoichi Chiba, Yasunori Sugiyama, Nozomu Nishi, Wakako Nonaka, Ryuta Murakami, Masaki Ueno
Sodium/glucose cotransporter 2 is expressed in choroid plexus epithelial cells and ependymal cells in human and mouse brains
Neuropathology, (2020)

Hironobu Ishiyama, Ryo C. Yanagita
*, Kazune Takemoto, Katsuya Kobashi, Yasunori Sugiyama,Yasuhiro Kawanami
Development of a D-allose-6-phosphate derivative with anti-proliferative activity against a human leukemia MOLT-4F cell line
Carbohydrate Research, 487, 107859 (2020)

[2019年]

Tatsuto Nakane, Ayae Ido, Takuma Higuchi, Hiroshi Todaka, Keiko Morisawa, Tadashi Nagamine, Kensaku Fukunaga, Shuji Sakamoto, Koji Murao, Yasunori Sugiyama*
Candidate plasticity gene 16 mediates suppression of insulin gene expression in rat insulinoma INS-1 cells under glucotoxic conditions
Biochemical and Biophysical Research Communications, 512, 189-195 (2019)

Yuuki Uezato, Isamu Kameshita, Keiko Morisawa, Shuji Sakamoto, Eiji Kinoshita, Emiko
Kinoshita-Kikuta, Tohru Koike, Yasunori Sugiyama
*
A method for profiling the phosphorylation state of tyrosine protein kinases
Biochimica et Biophysica Acta, 1876, 71-75 (2019)

[2018年]
Takuma Higuchi, Keiko Morisawa, Hiroshi Todaka, Sylvia Lai, Eunsup Chi, Kazutsugu Matsukawa, Yasunori Sugiyama, Shuji Sakamoto*
A negative feedback loop between nuclear factor 90 (NF90) and an anti-oncogenic microRNA, miR-7
Biochemical and Biophysical Research Communications, 503, 1819-1824 (2018)

Kazutoshi Akizuki, Taku Toyama, Masashi Yamashita, Yasunori Sugiyama, Atsuhiko Ishida, Isamu Kameshita, Noriyuki Sueyoshi*
Facile preparation of highly active casein kinase 1 using Escherichia coli constitutively expressing lambda phosphatase
Analytical Biochemistry, 549, 99-106 (2018)

杉山 康憲
*、上里 裕樹、亀下
マルチPK抗体を用いたプロテインキナーゼ研究の新展開
電気泳動、62, 5-8 (2018)

Jingya Lyu, Hitomi Imachi, Takuo Yoshimoto, Kensaku Fukunaga, Seisuke Sato, Tomohiro Ibata, Toshihiro Kobayashi, Tao Dong, Kazuko Yonezaki, Nao Yamaji, Fumi Kikuchi, Hisakazu Iwama, Ryou Ishikawa, Reiji Haba, Yasunori Sugiyama, Huanxiang Zhang
*, Koji Murao*
Thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic b cell line, INS-1 cells
Scientific Reports, 8, 1986 (2018)

[2017年]
杉山
康憲
*、亀下
マルチPK抗体を利用したプロテインキナーゼ研究
電気泳動、61, 29-33 (2017)

Yasunori Sugiyama*, Isamu Kameshita
Multi-PK antibodies: Powerful analytical tools to explore the protein kinase world
Biochemistry and Biophysics Reports, 11, 40-45 (2017)

Ami Oi, Syouichi Katayama, Naoya Hatano, Yasunori Sugiyama, Isamu Kameshita, Noriyuki Sueyoshi*
Subcellular distribution of cyclin-dependent kinase-like 5 (CDKL5) is regulated through phosphorylation by dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A)
Biochemical and Biophysical Research Communications, 482, 239-245 (2017)

[2016年]
Yasunori Sugiyama*, Sho Yamashita, Yuuki Uezato, Yukako Senga, Syouichi Katayama, Naoki Goshima, Yasushi Shigeri, Noriyuki Sueyoshi, Isamu Kameshita
Phosphorylated TandeMBP: A unique protein substrate for protein phosphatase assay
Analytical Biochemistry, 513, 47-53 (2016)

Takuma Higuchi, Hiroshi Todaka, Yasunori Sugiyama, Masafumi Ono, Nobuyuki Tamaki, Etsuro Hatano, Yuka Takezaki, Kazuhiro Hanazaki, Takeshi Miwa, Lai Sylvia Chin See, Keiko Morisawa, Masayuki Tsuda, Taketoshi Taniguchi, Shuji Sakamoto*
Suppression of miR-7 biogenesis by NF90-NF45 controls cell proliferation in hepatocellular carcinoma
Journal of Biological Chemistry, 291, 21074-21084 (2016)


Syouichi Katayama,
Yukako Senga, Ami Oi, Yosuke Miki, Yasunori Sugiyama, Noriyuki Sueyoshi, Isamu Kameshita*

Expression analyses of splice variants of zebrafish cyclin-dependent kinase-like 5 and its substrate, amphiphysin 1
Gene, 583, 15-23 (2016)

[2015年]
Yasunori Sugiyama*
, Syouichi Katayama, Isamu Kameshita, Keiko Morisawa, Takuma Higuchi, Hiroshi Todaka, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, Taketoshi Taniguchi, Shuji Sakamoto
Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE
MethodsX, 2, 469-474 (2015)

杉山 康憲*、亀下 勇、坂本 修士

マルチPK抗体とPhos-tagを利用した細胞内リン酸化動態の新規解析法
電気泳動、
59(2), 82-84 (2015)

Naohiro Kon, Yasunori Sugiyama, Hikari Yoshitane, Isamu Kameshita, Yoshitaka Fukada*

Cell-based inhibitor screening identifies multiple protein kinases important for circadian clock oscillations
Communicative & Integrative Biology, 8(4), e982405 (2015)


Hiroshi Todaka, Takuma Higuchi, Ken-ichi Yagyu, Yasunori Sugiyama, Fumika Yamaguchi, Keiko Morisawa, Masafumi Ono, Atsuki Fukushima, Masayuki Tsuda, Taketoshi Taniguchi, Shuji Sakamoto*
Overexpression of NF90-NF45 represses myogenic microRNA biogenesis, developing skeletal muscle atrophy and centronuclear muscle fibers
Molecular and Cellular Biology, 35(13), 2295-2308 (2015)


[2014年] 杉山研究室発足
杉山 康憲*、片山 将一、馬場 裕美、末吉 紀行、亀下 勇
液相等電点泳動を用いた二次元電位泳動法の活用
生物物理化学‐電気泳動‐、
58(2), 56-58 (2014)

Naohiro Kon, Tomoko Yoshikawa, Sato Honma, Yoko Yamagata, Hikari Yoshitane, Kimiko Shimizu, Yasunori Sugiyama, Chihiro Hara, Isamu Kameshita, Ken-ichi Honma, Yoshitaka Fukada*
CaMKII is essential for the cellular clock and coupling between morning and evening behavioral rhythms
Genes & Development 28(10), 1101-1110 (2014)


[2012年]
Takuma Higuchi, Shuji Sakamoto*, Yoshihiko Kakinuma, Shoko Kai, Ken-ichi Yagyu, Hiroshi Todaka, Eunsup Chi, Shoshiro Okada, Takako Ujihara, Keiko Morisawa, Masafumi Ono, Yasunori Sugiyama, Waka Ishida, Atsuki Fukushima, Masayuki Tsuda, Yasutoshi Agata, Taketoshi Taniguchi
High expression of nuclear factor 90 (NF90) leads to mitochondrial degradation in skeletal and cardiac muscles
PLoS ONE 7(8), e43340 (2012)


Syouichi Katayama, Yasunori Sugiyama, Naoya Hatano, Toru Terachi, Noriyuki Sueyoshi, Isamu Kameshita*
PKL01, an Ndr kinase homologue in plant, shows tyrosine kinase activity
Journal of Biochemistry 152(4), 347-353 (2012)

Eiji Kinoshita*, Emiko Kinoshita-Kikuta, Yasunori Sugiyama, Yoshitaka Fukada, Tomomitsu Ozeki, Tohru Koike
Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin
Proteomics 12(7), 932-937 (2012)


[2011年]
Keisuke Kaneko, Yasunori Sugiyama, Yusuke Yamada, Noriyuki Sueyoshi, Akira Watanabe, Yasuhiko Asada, Atsuhiko Ishida, Isamu Kameshita*
CoPK32 is a novel stress-responsive protein kinase in the mushroom Coprinopsis cinerea
Biochimica et Biophysica Acta 1810(6), 620-629 (2011)


Yasunori Sugiyama, Koji Murao*, Hitomi Imachi, Noriyuki Sueyoshi, Toshihiko Ishida, Isamu Kameshita*
Calcium/calmodulin-dependent protein kinase IV involvement in the pathophysiology of glucotoxicity in rat pancreatic
b-cells
Metabolism 60(1), 145-153 (2011)


[2010年]
Yasunori Sugiyama, Naoya Hatano, Noriyuki Sueyoshi, Isao Suetake, Shoji Tajima, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, Isamu Kameshita*
The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1
d/e
Biochemical journal 427(3), 489-497 (2010)


Takaki Nimura, Yasunori Sugiyama, Noriyuki Sueyoshi, Yasushi Shigeri, Atsuhiko Ishida, Isamu Kameshita*
A minimum size homologue of Ca2+/calmodulin-dependent protein kinase IV naturally occurring in zebrafish
Journal of Biochemistry 147(6), 857-865 (2010)

[2008年]
Isamu Kameshita*, Mari Sekiguchi, Daisuke Hamasaki, Yasunori Sugiyama, Naoya Hatano, Isao Suetake, Shoji Tajima, Noriyuki Sueyoshi
Cyclin-dependent kinase-like 5 binds and phosphorylates DNA methyltransferase 1
Biochemical and Biophysical Research Communications 377(4), 1162-1167 (2008)


Yasunori Sugiyama, Atsuhiko Ishida, Noriyuki Sueyoshi, Isamu Kameshita*
Tyrosine kinase activity of a Ca2+/calmodulin-dependent protein kinase II catalytic fragment
Biochemical and Biophysical Research Communications 377(2), 648-652 (2008)


Yasunori Sugiyama, Sachiko Shimomura, Noriyuki Sueyoshi, Isamu Kameshita*
Two-dimensional gel electrophoretic analysis of cyanogen bromide fragments containing subdomain VIB of protein kinases using a Multi-PK antibody
Analytical Biochemistry 373(1), 173-175 (2008)


[2007年]
Noriyuki Sueyoshi, Toshihiko Takao, Takaki Nimura, Yasunori Sugiyama, Takamasa Numano, Yasushi Shigeri, Takanobu Taniguchi, Isamu Kameshita*, Atsuhiko Ishida*
Inhibitors of the Ca2+/calmodulin-dependent protein kinase phosphatase family (CaMKP and CaMKP-N)
Biochemical and Biophysical Research Communications 363(3), 715-721 (2007)


Isamu Kameshita*, Yusuke Yamada, Tetsuyuki Nishida, Yasunori Sugiyama, Noriyuki Sueyoshi, Akira Watanabe, Yasuhiko Asada
Involvement of Ca2+/calmodulin-dependent protein kinases in mycelial growth of the basidiomycetous mushroom, Coprinus cinereus
Biochimica et Biophysica Acta 1770(9), 1395-1403 (2007)


[2006年]
Yasunori Sugiyama, Noriyuki Sueyoshi, Isamu Kameshita*
Two-dimensional expression pattern analysis of protein kinases after separation by MicroRotofor/SDS–PAGE
Analytical Biochemistry 359(2), 271-273 (2006)


[2005年]
Yasunori Sugiyama, Noriyuki Sueyoshi, Yasushi Shigeri, Yoshiro Tatsu, Noboru Yumoto, Atsuhiko Ishida, Takanobu Taniguchi, Isamu Kameshita*
Generation and application of a monoclonal antibody that detects a wide variety of protein tyrosine kinases
Analytical Biochemistry 347(1), 112-120 (2005)


Isamu Kameshita*, Tetsuyuki Nishida, Satoko Nakamura, Yasunori Sugiyama, Noriyuki Sueyoshi, Yosuke Umehara, Mika Nomura, Shigeyuki Tajima
Expression Cloning of a Variety of Novel Protein Kinases in Lotus japonicus
Journal of Biochemistry 137(1), 33-39 (2005)


<著書>
有澤 琴子、馬谷 千恵、大上 雅史、小野田 淳人、香川 璃奈、杉山 康憲、高橋 裕佳、瀧 慎太郎、道喜 慎太郎、豊田 優、藤原 慶(五十音順
これだけ! 生化学(
6章および7章)
秀和システム、
145-224 (2014)

<特許>
杉山 康憲末吉 紀行、亀下 勇、茂里 康、達 吉郎湯元 昇、石田 敦彦、谷口 隆信
抗チロシンキナーゼ抗体およびその利

出願、特願2005-190494、特許第4956816 (2012)

<受賞>
61回日本生化学会中国・四国支部例会学術奨励賞、20207月(中根達人)
日本電気泳動学会奨励賞(服部賞)、201711(杉山康憲)
57回日本生化学会中国・四国支部例会学術奨励賞、20165月(井戸彩詠)
47回日本生化学会中国・四国支部例会学術奨励賞、20065月(杉山康憲)

<その他>
杉山 康憲
Phos-tag SDS-PAGEを利用したDNAメチルトランスフェラーゼ1をリン酸化するプロテインキナーゼの同定
Wako Web セミナー(ここ

杉山 康憲
Phos-tag gel (Phos-tag gel の作製方法を紹介しています。)
Wako Web セミナー(ここ